Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25455
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Canine, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1794 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein of human Erythropoietin/EPO
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free
Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (NBP3-25455) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1793] (NBP3-25454) (1 ug/mL) Please notice that NBP3-25454 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25455. Please contact us for custom HRP-conjugated antibody.
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] -
ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (NBP3-25454) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1794] (NBP3-25455) (1 ug/mL) Please notice that NBP3-25455 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25454. Please contact us for custom HRP-conjugated antibody.
ELISA: Erythropoietin/EPO Antibody (HL1794) [NBP3-25455] -
Sandwich ELISA detection of recombinant (CHO cells expressed, full-length human EPO protein) using antibodies as below.Capture: EPO antibody [HL1793] (5 ug/mL)
Detection: HRP-conjugated EPO antibody [HL1794] (NBP3-25455-01) (1 ug/mL
Applications for Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Sandwich ELISA
Assay dependent
Western Blot
Assay dependent
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Erythropoietin/EPO
Alternate Names
ECYT5, EPO, MVCD2
Gene Symbol
EPO
Additional Erythropoietin/EPO Products
Product Documents for Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free
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Q:
Does the human Erythropoietin (Epo) polyclonal antibody (Catalog # AB-286-NA) bind Epo if the receptor is not present?
A: AB-286-NA binds Epo if the receptor is not present.
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Associated Pathways
Jak/STAT Signaling Pathway
