ETFB Antibody

Novus Biologicals | Catalog # NBP1-49844

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ETFB Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PQRTAGVKVETTE corresponding to C-Terminus according to NP_001014763.1.

Epitope

PQRTAGVKVETTE - C Terminus

Specificity

This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ETFB Antibody

Western Blot: ETFB Antibody [NBP1-49844]

Western Blot: ETFB Antibody [NBP1-49844]

Western Blot: ETFB Antibody [NBP1-49844] - Analysis of ETFB (aa 231-243) in Mouse Liver lysate (35ug protein in RIPA buffer) using NBP1-49844 at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ETFB Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in human, mouse and rat liver lysates (calculated MW of 27.8 kDa band according to NP_001976.1). IHC-P: Human liver shows mitochondrial staining in hepatocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ETFB

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene.

Alternate Names

beta-ETF, electron transfer flavoprotein beta subunit, electron transfer flavoprotein beta-subunit, electron transfer flavoprotein subunit beta, electron transfer flavoprotein, beta polypeptide, electron-transfer-flavoprotein, beta polypeptide, electron-transferring-flavoprotein, beta polypeptide, MADD

Entrez Gene IDs

2109 (Human); 110826 (Mouse); 292845 (Rat)

Gene Symbol

ETFB

UniProt

NP_001976.1 (Human)

Additional ETFB Products

Product Documents for ETFB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ETFB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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