ETV6/Tel Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80695

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot, ELISA, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ETV6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQPEVILHQNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ETV6/Tel Antibody - BSA Free

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining in human breast and kidney tissues using anti-ETV6 antibody. Corresponding ETV6 RNA-seq data are presented for the same tissues.
Western Blot: ETV6/Tel Antibody [NBP1-80695]

Western Blot: ETV6/Tel Antibody [NBP1-80695]

Western Blot: ETV6/Tel Antibody [NBP1-80695] - Analysis in human cell lines U2OS and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ETV6/Tel Antibody [NBP1-80695]

Immunocytochemistry/ Immunofluorescence: ETV6/Tel Antibody [NBP1-80695]

Immunocytochemistry/Immunofluorescence: ETV6/Tel Antibody [NBP1-80695] - Staining of human cell line U-2 OS shows localization to nucleoli & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining of human breast shows high expression.
Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695]

Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining of human skin shows weak nuclear positivity in keratinocytes.
ETV6/Tel Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ETV6/Tel Antibody - BSA Free [NBP1-80695]

Chromatin Immunoprecipitation-exo-Seq: ETV6/Tel Antibody - BSA Free [NBP1-80695]

ChIP-Exo-Seq composite graph for Anti-ETV6 (NBP1-80695) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for ETV6/Tel Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ETV6

Tel encodes an ETS family transcription factor. The product of this gene contains two functional domains: a N terminal pointed (PNT) domain that is involved in the protein protein interactions with itself and other proteins, and a C terminal DNA binding domain. Gene knockout studies in mice suggest that it is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma.

Long Name

ETS Translocation Variant 6

Alternate Names

TEL, TEL/ABL

Entrez Gene IDs

2120 (Human)

Gene Symbol

ETV6

UniProt

P41212 (Human)

Additional ETV6 Products

Product Documents for ETV6/Tel Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ETV6/Tel Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ETV6/Tel Antibody - BSA Free

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Protocols

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