EWSR1 Antibody (5H7) - BSA Free

Knockdown Validated: EWSR1 Antibody (5H7) - BSA Free [NBP1-92686] -

Effects of siRNA-mediated knockdown of expression of AldoA, EWSR1 and ILF3-90 on enterovirus replication.A, B. HeLa cells were transfected with siRNAs specific to AldoA, EWSR1 and 90KDa isoform of ILF3, or non-targeting control siRNA, and polio or Coxsakie B3 replicon replication assays were performed 72 h post siRNA transfection. The total replication signal was calculated as the area under the corresponding kinetics curves. Cell viability signal is proportional to the level of ATP in cells. Western blots show the efficacy of siRNA-mediated knockdown of the targeted proteins. C. HeLa cells were transfected with siRNAs specific to AldoA, EWSR1 and 90KDa isoform of ILF3, or non-targeting control siRNA. 72 h post siRNA transfection cells were infected with an MOI of 1 PFU/cell of poliovirus or Coxsackie virus B3, and the total virus yield was determined at 6 h p. i. Western blots show the efficacy of siRNA-mediated knockdown of the targeted proteins. D. HeLa cells were transfected with siRNAs specific to 90KDa isoform of ILF3 or EWSR1 or a non-targeting control siRNA. 72h post siRNA transfection, the cells were transfected with a replication-defective replicon RNA containing the delta 3D mutation. The total translation signal was calculated as the area ander the curve from 1 to 4 h post delta 3D RNA transfection. Western blots show the efficacy of siRNA-mediated knockdown of the targeted proteins. E. HeLa cells were transfected with siRNAs specific to AldoA, EWSR1 and 90KDa isoform of ILF3, or non-targeting control siRNA, and cell viability assays detecting the level of ATP or the activity of the mitochondrial respiratory chain enzymes were performed 72h post siRNA transfection. Western blots show the efficacy of siRNA-mediated knockdown of the targeted proteins. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36306280), licensed under a CC-BY license. Not internally tested by Novus Biologicals.