Exosome Detection (Western Blot) Antibody Pack

Novus Biologicals | Catalog # NBP3-11740

Novus Biologicals
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Key Product Details

Species

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Product Summary for Exosome Detection (Western Blot) Antibody Pack

This pack contains 1 vial each of: NBP2-77452SS (0.025 mg); MAB1663-SP (25 ug); MAB25292-SP (25 ug); NB100-65805SS (0.025 ml); NBP2-42225SS (0.025 ml); NBP1-47546SS (0.025 ml); MAB1455-SP (25 ug), HAF007 and HAF008.

Product Specifications

Specificity

Please see each vial's data sheet for specificity information specific to each product.

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Exosome Detection (Western Blot) Antibody Pack

Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740]

Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740]

Exosome-Detection-Western-Blot-Antibody-Pack-Western-Blot-NBP3-11740-img0001.jpg
Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740]

Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740]

Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Western blot shows lysates of TIME human endothelial cell line and human platelets. PVDF membrane was probed with 2 ug/mL of Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB25292) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD9 at approximately 24 kDa (as indicated).
Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 ug/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated).

Kit Contents for Exosome Detection (Western Blot) Antibody Pack

Formulation, Preparation, and Storage

Formulation

See individual datasheets

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage is content dependent.

Background: Exosome Detection (Western Blot)

The Exosome Detection (Western Blot) Antibody Pack contains antibodies from each category recommended by the International Society for Extracellular Vesicles (ISEV) for characterization of extracellular vesicles (EVs), with data on exosomes published in peer-reviewed journals. It includes three tetraspanins (CD9, CD63, and CD81), one intracellular protein associated with multivesicular body (MVB) formation (TSG101), and one chaperone protein (HSP70). The pack also contains two antibodies to determine the degree of contamination of exosome prep with secretory vesicles (Calnexin) and circulating free protein (albumin). All antibodies have been validation for use in western blotting.

Alternate Names

EV detection, Exosome Markers, extracellular vesicle detection

Additional Exosome Detection (Western Blot) Products

Product Documents for Exosome Detection (Western Blot) Antibody Pack

Certificate of Analysis

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Product Specific Notices for Exosome Detection (Western Blot) Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Protocols

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