Exportin 4 Antibody - BSA Free

Immunoprecipitation: Exportin 4 Antibody [NB100-56495] -

Immunoprecipitation: Exportin 4 Antibody [NB100-56495] - RASSF1A binds to XPO6 though its SARAH domainCo‐immunoprecipitation of endogenous RASSF1A with endogenous RAN, CRM1/XPO1, XPO4, XPO5, XPO6 & XPO7 from HeLa cell lysates, compared with the IgG control.Upper: graphical representation of the domain structure of full‐length RASSF1A & mutant constructs used for mapping RASSF1A/XPO6 interaction. Different domains are abbreviated as follows: SARAH, Salvador‐RASSF‐Hippo domain; C1, N‐terminal C1 type zinc fingers; & RA, Ras‐binding domain. Lower: Western blot analysis of MYC immunoprecipitation from the indicated inputs from HeLa cells. Co‐immunoprecipitation of endogenous XPO6 with RAN in siRASSF1A from HeLa cell lysates.Co‐immunoprecipitation of endogenous XPO6 with endogenous RAN, RASSF1A & MST2 in siRASSF1A & siMST2 HeLa cells. Quantification of the interaction of XPO6 with RASSF1A, RAN & MST2 relative to XPO6 is shown. Error bars derive from three independent experiments & represent the SEM.Co‐immunoprecipitation of endogenous RASSF1A with endogenous XPO6 & RAN in siRNA‐mediated knockdown of MST2 HeLa cells. Quantification of the interaction of RASSF1A with XPO6, RAN & MST2 relative to RASSF1A is shown. Error bars derive from three independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.