![Western Blot: EYA3 Antibody [NBP1-46818]](https://resources.rndsystems.com/images/products/EYA3-Antibody-Western-Blot-NBP1-46818-img0002.jpg "Western Blot: EYA3 Antibody [NBP1-46818]")
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1 and 30 of human eyes absent homolog 3 using the numbering given in entry NP_001981.2 (GeneID 2140).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EYA3 Antibody
Western Blot: EYA3 Antibody [NBP1-46818]
Western Blot: EYA3 Antibody [NBP1-46818] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46818 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46819, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).![Immunohistochemistry: EYA3 Antibody [NBP1-46818]](https://resources.rndsystems.com/images/products/EYA3-Antibody-Immunohistochemistry-NBP1-46818-img0006.jpg "Immunohistochemistry: EYA3 Antibody [NBP1-46818]")
Immunohistochemistry: EYA3 Antibody [NBP1-46818]
Immunohistochemistry: EYA3 Antibody [NBP1-46818] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA3 used at a dilution of 1:200 (1ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818]](https://resources.rndsystems.com/images/products/EYA3-Antibody-Immunohistochemistry-Paraffin-NBP1-46818-img0005.jpg "Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818]")
Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818]
Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818] - FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.Applications for EYA3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EYA3
Alternate Names
DKFZp686C132, EC 3.1.3.48, eyes absent (Drosophila) homolog 3, eyes absent 3, eyes absent homolog 3, eyes absent homolog 3 (Drosophila)
Gene Symbol
EYA3
Additional EYA3 Products
Product Documents for EYA3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EYA3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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