EYA4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85547

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EYA4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TGGENMTVLNTADWLLSCNTPSSATMSLLAVKTEPLNSSETTATTGDGALDTFTGSVITSSGYSPRSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA4 Antibody - BSA Free

Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547] - analysis in human cell lines HeLa and MCF-7 using Anti-EYA4 antibody. Corresponding EYA4 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-85547]

Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-85547]

Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-85547] - Staining of human heart muscle shows distinct cytoplasmic positivity in myocytes.

Applications for EYA4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EYA4

EYA4 is thought to play a role in transcription regulation during organogenesis through its intrinsic protein phosphatase activity. It may be involved in development of the eye.

Alternate Names

CMD1J, DFNA10, dJ78N10.1 (eyes absent), EC 3.1.3.48, eyes absent (Drosophila) homolog 4, eyes absent homolog 4, eyes absent homolog 4 (Drosophila)

Gene Symbol

EYA4

Additional EYA4 Products

Product Documents for EYA4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EYA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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