EZH2/KMT6 Antibody (9Q2R9)

Novus Biologicals | Catalog # NBP3-15783

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Q2R9 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 148-272 of human EZH2/KMT6 (Q15910). ELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EZH2/KMT6 Antibody (9Q2R9)

EZH2/KMT6 Antibody (9Q2R9)

Western Blot: EZH2/KMT6 Antibody (9Q2R9) [NBP3-15783] -

Western Blot: EZH2/KMT6 Antibody (9Q2R9) [NBP3-15783] - analysis of extracts of various cell lines, using EZH2/KMT6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.
EZH2/KMT6 Antibody (9Q2R9)

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] -

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using EZH2/KMT6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
EZH2/KMT6 Antibody (9Q2R9)

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] -

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using EZH2/KMT6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
EZH2/KMT6 Antibody (9Q2R9)

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] -

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using EZH2/KMT6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
EZH2/KMT6 Antibody (9Q2R9)

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] -

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using EZH2/KMT6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
EZH2/KMT6 Antibody (9Q2R9)

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] -

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using EZH2/KMT6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
EZH2/KMT6 Antibody (9Q2R9)

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] -

Immunohistochemistry: EZH2/KMT6 Antibody (9Q2R9) [EZH2/KMT6] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using EZH2/KMT6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for EZH2/KMT6 Antibody (9Q2R9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EZH2

KMT6 / EZH2 encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Enhancer of Zeste Homolog 2 [Drosophila]

Alternate Names

ENX1, KMT6

Gene Symbol

EZH2

Additional EZH2 Products

Product Documents for EZH2/KMT6 Antibody (9Q2R9)

Certificate of Analysis

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Product Specific Notices for EZH2/KMT6 Antibody (9Q2R9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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