EZH2/KMT6 Antibody - BSA Free

Western Blot: EZH2/KMT6 Antibody [NBP2-59251]

Western Blot: EZH2/KMT6 Antibody [NBP2-59251] - Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) and crude serum directed against EZH2 at 1:1,000 in TBS-Tween + 5% skimmed milk. The antibody recognizes endogenous EZH2 in HeLa nuclear extract. On the right side, a molecular weight marker is shown (in kDa). The arrow indicates the location of the protein of interest.

Chromatin Immunoprecipitation: EZH2/KMT6 Antibody [NBP2-59251] - ChIP assays were performed using Jurkat cells (human peripheral blood leukemia T-cells), the polyclonal antibody directed against EZH2 and the PCR primer set MYT1. IgG was used as negative control. Decreasing amounts of input were used for end-point PCR (lanes 1-3). The positive IP with the antibody anti-EZH2 gives a clear signal (lane 5), while the negative control gives no signal (lane 4). The figure shows the results for IgG purified antibody (2 ug/ChIP), however the crude serum also works in ChIP at 5 ul/ChIP.