Ezrin Antibody (CL2384) - BSA Free

Novus Biologicals | Catalog # NBP2-36760

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2384

Format

BSA Free
View all Ezrin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P15311, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ezrin Antibody (CL2384) - BSA Free

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in human small intestine and skeletal muscle tissues. Corresponding EZR RNA-seq data are presented for the same tissues.
Western Blot: Ezrin Antibody (CL2384) [NBP2-36760]

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760]

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Analysis of extracts from U-251 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-EZR monoclonal antibody.Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in U251 cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Western Blot: Ezrin Antibody (CL2384) [NBP2-36760]

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760]

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in HeLa cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human cell line A431 showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in MCF7 cell line showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760]

Immunocytochemistry/Immunofluorescence: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in U2OS cell line with Anti-EZR monoclonal antibody, showing specific staining of plasma membrane in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.
Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human placenta shows strong positivity in apical membrane in trophoblastic cells.
Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human tonsil shows strong membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760]

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Applications for Ezrin Antibody (CL2384) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ezrin

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Alternate Names

CVIL, CVL, Cytovillin 2, EZR, p81, VIL2, Villin 2

Gene Symbol

EZR

UniProt

P15311 (Human)

Additional Ezrin Products

Product Documents for Ezrin Antibody (CL2384) - BSA Free

Certificate of Analysis

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Product Specific Notices for Ezrin Antibody (CL2384) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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