FAAH Antibody (10A1M7)

Name: FAAH Antibody (10A1M7)
Brand: Novus Biologicals
SKU: NBP3-16330
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16330

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10A1M7 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 480-579 of human FAAH (O00519). DLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FAAH Antibody (10A1M7)

Western Blot: FAAH Antibody (10A1M7) [NBP3-16330]

Western Blot: FAAH Antibody (10A1M7) [NBP3-16330] - Western blot analysis of extracts of various cell lines, using FAAH Rabbit mAb (NBP3-16330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/ Immunofluorescence: FAAH Antibody (10A1M7) [NBP3-16330] -

Immunocytochemistry/ Immunofluorescence: FAAH Antibody (10A1M7) [NBP3-16330] - Confocal imaging of NIH/3T3 cells using FAAH Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

FAAH Antibody (10A1M7) [NBP3-16330] -

Analysis of various lysates using FAAH1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.

Applications for FAAH Antibody (10A1M7)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:200 - 1:800

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FAAH

FAAH encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide.

Long Name

Fatty Acid Amide Hydrolase

Alternate Names

FAAH-1, FAAH1, PSAB

Gene Symbol

FAAH

Additional FAAH Products

Product Documents for FAAH Antibody (10A1M7)

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Certificate of Analysis

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Product Specific Notices for FAAH Antibody (10A1M7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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