FABP2/I-FABP Antibody (5W9L6)

Novus Biologicals | Catalog # NBP3-16717

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5W9L6 expressed in HEK293
View all FABP2/I-FABP Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FABP2/I-FABP (P12104). MAFDSTWKVDRSENYDKFMEKMGVNIVKRKLAAHDNLKLTITQEGNKFTVKESSAFRNIEVVFELGVTFNYNLADGTELRGTWSLEGNKLIGKFKRTDNG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FABP2/I-FABP Antibody (5W9L6)

Western Blot: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Western Blot: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Western Blot: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717] - Western blot analysis of extracts of various cell lines, using FABP2/I-FABP Rabbit mAb (NBP3-16717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Immunohistochemistry: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Immunohistochemistry: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717] - Immunofluorescence analysis of mouse intestin using FABP2/I-FABP Rabbit mAb (NBP3-16717) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717] - Immunohistochemistry of paraffin-embedded rat intestine using FABP2/I-FABP Rabbit mAb (NBP3-16717) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717] - Immunohistochemistry of paraffin-embedded mouse intestin using FABP2/I-FABP Rabbit mAb (NBP3-16717) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
FABP2/I-FABP Antibody (5W9L6)

Immunocytochemistry/ Immunofluorescence: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717] -

Immunocytochemistry/ Immunofluorescence: FABP2/I-FABP Antibody (5W9L6) [NBP3-16717] - Confocal imaging of paraffin-embedded mouse small intestine using FABP2/I-FABP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

Applications for FABP2/I-FABP Antibody (5W9L6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FABP2/I-FABP

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Gene Symbol

FABP2

Additional FABP2/I-FABP Products

Product Documents for FABP2/I-FABP Antibody (5W9L6)

Certificate of Analysis

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Product Specific Notices for FABP2/I-FABP Antibody (5W9L6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Early Endodermal Lineage Markers
Obesity

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Protocols

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