FACL4 Antibody (4W3T9)

Novus Biologicals | Catalog # NBP3-16012

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4W3T9 expressed in HEK293
View all FACL4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human FACL4 (NP_075266.1). MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FACL4 Antibody (4W3T9)

Immunoprecipitation: FACL4 Antibody (4W3T9) [NBP3-16012]

Immunoprecipitation: FACL4 Antibody (4W3T9) [NBP3-16012]

Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.
FACL4 Antibody (4W3T9)

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] -

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
FACL4 Antibody (4W3T9)

Western Blot: FACL4 Antibody (4W3T9) [FACL4] -

Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of various lysates using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
FACL4 Antibody (4W3T9)

Western Blot: FACL4 Antibody (4W3T9) [FACL4] -

Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Rat liver using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
FACL4 Antibody (4W3T9)

Western Blot: FACL4 Antibody (4W3T9) [FACL4] -

Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Hep G2 cells using FACL4 Rabbit mAb at 1:58000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
FACL4 Antibody (4W3T9)

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (4W3T9) [FACL4] -

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (4W3T9) [FACL4] - Confocal imaging of Hep G2 cells using FACL4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
FACL4 Antibody (4W3T9)

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] -

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Rat brain using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
FACL4 Antibody (4W3T9)

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] -

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
FACL4 Antibody (4W3T9)

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] -

Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Applications for FACL4 Antibody (4W3T9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:10000 - 1:20000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FACL4

The protein encoded by the FACL4 gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Althoughdiffering in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this familyconvert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis andfatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme maycontribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcriptvariants. (provided by RefSeq)

Alternate Names

ACS4mental retardation, X-linked 68, acyl-CoA synthetase 4, acyl-CoA synthetase long-chain family member 4, EC 6.2.1.3, FACL4long-chain 4, LACS 4, LACS4MRX68, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4, long-chain-fatty-acid--CoA ligase 4, mental retardation, X-linked 63, MRX63

Gene Symbol

ACSL4

Additional FACL4 Products

Product Documents for FACL4 Antibody (4W3T9)

Certificate of Analysis

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Product Specific Notices for FACL4 Antibody (4W3T9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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