FACL4 Antibody (HL229) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13666

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat, Drosophila

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL229 expressed in HEK293

Format

Azide and BSA Free
View all FACL4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for FACL4 Antibody (HL229) - Azide and BSA Free

Western Blot: FACL4 Antibody (HL229) [NBP3-13666]

Western Blot: FACL4 Antibody (HL229) [NBP3-13666]

Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666]

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666]

Immunocytochemistry/Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666]

Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666]

Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: FACL4 Antibody (HL229) [NBP3-13666]

Western Blot: FACL4 Antibody (HL229) [NBP3-13666]

Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.
Western Blot: FACL4 Antibody (HL229) [NBP3-13666]

Western Blot: FACL4 Antibody (HL229) [NBP3-13666]

Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.
FACL4 Antibody (HL229)

Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] -

Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL44] detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FACL4 stained by FACL4 antibody [HL44] (NBP3-13666) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FACL4 Antibody (HL229)

Western Blot: FACL4 Antibody (HL229) [NBP3-13666] -

Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Drosophila tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
FACL4 Antibody (HL229)

Western Blot: FACL4 Antibody (HL229) [NBP3-13666] -

Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for FACL4 Antibody (HL229) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FACL4

The protein encoded by the FACL4 gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Althoughdiffering in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this familyconvert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis andfatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme maycontribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcriptvariants. (provided by RefSeq)

Alternate Names

ACS4mental retardation, X-linked 68, acyl-CoA synthetase 4, acyl-CoA synthetase long-chain family member 4, EC 6.2.1.3, FACL4long-chain 4, LACS 4, LACS4MRX68, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4, long-chain-fatty-acid--CoA ligase 4, mental retardation, X-linked 63, MRX63

Gene Symbol

ACSL4

Additional FACL4 Products

Product Documents for FACL4 Antibody (HL229) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FACL4 Antibody (HL229) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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