FACL4 Antibody

Novus Biologicals | Catalog # NB300-861

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FACL4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HYLKDIERMYGGK corresponding to C-Terminus according to NP_075266.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FACL4 Antibody

Western Blot: FACL4 Antibody [NB300-861]

Western Blot: FACL4 Antibody [NB300-861]

Western Blot: FACL4 Antibody [NB300-861] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NB300-861]

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NB300-861]

Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NB300-861] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861]

Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861]

Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: FACL4 Antibody [NB300-861]

Flow Cytometry: FACL4 Antibody [NB300-861]

Flow Cytometry: FACL4 Antibody [NB300-861] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861]

Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861]

Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for FACL4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 80 kDa band observed in HepG2 lysates (calculated MW of 79.2 kDa band according to NP_075266.1).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FACL4

The protein encoded by the FACL4 gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Althoughdiffering in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this familyconvert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis andfatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme maycontribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcriptvariants. (provided by RefSeq)

Alternate Names

ACS4mental retardation, X-linked 68, acyl-CoA synthetase 4, acyl-CoA synthetase long-chain family member 4, EC 6.2.1.3, FACL4long-chain 4, LACS 4, LACS4MRX68, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4, long-chain-fatty-acid--CoA ligase 4, mental retardation, X-linked 63, MRX63

Entrez Gene IDs

2182 (Human); 50790 (Mouse); 113976 (Rat)

Gene Symbol

ACSL4

UniProt

NP_004449 (Human)

Additional FACL4 Products

Product Documents for FACL4 Antibody

Certificate of Analysis

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Product Specific Notices for FACL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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