FACL4 Antibody

Novus Biologicals | Catalog # NBP2-16401

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all FACL4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

Localization

Mitochondrion outer membrane; Single-pass type III membrane protein; Peroxisome membrane; Microsome membrane; Endoplasmic reticulum membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FACL4 Antibody

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - mouse kidney. FACL4 stained by FACL4 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: FACL4 Antibody [NBP2-16401]

Western Blot: FACL4 Antibody [NBP2-16401]

Western Blot: FACL4 Antibody [NBP2-16401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401]

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401]

Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16401] - analysis of FACL4 in MCF10A cells using anti-FACL4 antibody. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Rat middle brain. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401]

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Mouse lymph node. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation: FACL4 Antibody [NBP2-16401]

Immunoprecipitation: FACL4 Antibody [NBP2-16401]

Immunoprecipitation: FACL4 Antibody [NBP2-16401] - Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ug of FACL4 antibody [C3], C-term Western blot analysis was performed using FACL4 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
FACL4 Antibody

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] -

FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FACL4 Antibody

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] -

FACL4 antibody [C3], C-term detects FACL4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FACL4 Antibody

Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] -

FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

Expression and location of ferroptosis-related proteins in diabetic mice retinas. (A) Western blot analysis of ferroptosis-related proteins expression levels in retinas at 1 and 3 months post-diabetes. GAPDH was used as a control. (B) GPX4 and SLC7A11 protein expression was remarkably decreased in diabetic mice retinas compared with the control retinas. Expression of ACSL4, FTH1, and NCOA4 proteins was significantly increased in retinas at 1 and 3 months post-diabetes. (C) Immunofluorescence staining of location of ferroptosis-related proteins (red) and nuclear (blue) in retinas at 1 and 3 months post-diabetes: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS). Data are shown as mean +/- SEM, n = 3 per group for Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

Fer-1 treatment attenuated changes in ferroptosis-related proteins’ expression in HG-stimulated 661W cells after 18 h. (A) Western blot analysis of ferroptosis-related proteins’ expression levels in HG-induced 661W cells after Fer-1 treatment. GAPDH was used as a control. (B) The downregulation in GPX4 and SLC7A11 protein expression in HG-stimulated 661W cells was significantly attenuated after Fer-1 treatment. The upregulation in ACSL4, FTH1, and NCOA4 protein expression in HG-stimulated 661W cells was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after Fer-1 administration. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

Fer-1 administration attenuated changes of ferroptosis-related proteins expression in diabetic mice retinas. (A) Western blot analysis of ferroptosis-related proteins expression levels in diabetic mice retinas after Fer-1 treatment. GAPDH was used as a control. (B) The decrease in GPX4 and SLC7A11 protein expression in diabetic mice retinas was significantly attenuated after Fer-1 treatment. The increase in ACSL4, FTH1, and NCOA4 protein expression in diabetic mice retinas was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in diabetic mice retinas after Fer-1 treatment: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS). Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

Extraction ion-containing PMs (PM2.5 EI) cause ferroptosis in macrophages.A Western blot analysis after incubation of RAW264.7 cells with three types of PM (100 μg/ml) for 18 h. B Malondialdehyde (MDA) formation in RAW264.7 cells after incubation with 100 μg/ml PMs for 12 h, investigated using a lipid peroxidation assay kit. C Intracellular ferrous iron levels are detected in J774A.1 cells incubated with 50 μg/ml of PMs for 12 h. D WST-8 assay demonstrating the cell viability analysis in RAW264.7 line after pretreatment with ferrostatin-1 (Fer-1; 2 μM), liproxstatin-1 (Lip-1; 2 μM), or deferiprone (DFP; 100 μM) for 2 h followed by the stimulation with 100 μg/ml of PM2.5 EI for 24 h. E Western blot analysis using RAW264.7 cells after preincubation with Fer-1 (2 μM), Lip-1 (2 μM), or DFP (100 μM) for 2 h followed by stimulation with 100 μg/ml of PM2.5 EI for 24 h. F Detection of lipid peroxidation in terms of MDA in J774A.1 cells after preincubation with Fer-1 (2 μM), Lip-1 (2 μM), or DFP (100 μM) for 2 h followed by treatment using 50 μg/ml of PM2.5 EI for 12 h. All data are presented as the means +/- standard deviations from at least three independent experiments. *P < 0.05, **P < 0.01 and #P < 0.001. All experiments were conducted at least three times. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41420-024-01874-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FACL4 Antibody

Western Blot: FACL4 Antibody [NBP2-16401] -

PM2.5 EI induces a correlation between the activation of inflammasomes and the induction of ferroptosis.A and B Western blot analysis in J774A.1 cells after preincubation with CY-09 (10 μM) or YVAD (20 μM) for 2 h and subsequent stimulation with 50 μg/ml of PM2.5 EI for 24 h. C MDA levels in J774A.1 cells after pretreatment with CY-09 (10 μM) or YVAD (20 μM) for 2 h followed by stimulation with 50 μg/ml of PM2.5 EI for 12 h tested using a lipid peroxidation assay kit. D Western blot analysis using J774A.1 cells preincubated with Fer-1 (2 μM), Lip-1 (2 μM), or DFP (100 μM) for 2 h and treated using 50 μg/ml of PM2.5 EI for 24 h. E, F ELISA assay revealed the IL-1 beta and IL-18 levels in the medium of J774A.1 cell culture after pretreatment with CY-09 (10 μM) or YVAD (20 μM) for 2 h and subsequent stimulation with 50 μg/ml of PM2.5 EI for 12 h. All data are presented as the means +/- standard deviations from at least three independent experiments. *P < 0.05, **P < 0.01 and #P < 0.001. All experiments were conducted at least three times. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41420-024-01874-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FACL4 Antibody

Immunocytochemistry/ Immunofluorescence: FACL4 Antibody [NBP2-16401] -

HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FACL4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:1000-1:5000

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 4 using NBP2-16401 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FACL4

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Alternate Names

ACS4mental retardation, X-linked 68, acyl-CoA synthetase 4, acyl-CoA synthetase long-chain family member 4, EC 6.2.1.3, FACL4long-chain 4, LACS 4, LACS4MRX68, lignoceroyl-CoA synthase, Long-chain acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4, long-chain-fatty-acid--CoA ligase 4, mental retardation, X-linked 63, MRX63

Gene Symbol

ACSL4

UniProt

O60488 (Human)

Additional FACL4 Products

Product Documents for FACL4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FACL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for FACL4 Antibody (1)

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Mcf10A cell line
    Species: Human
    Verified Customer | Posted 04/03/2015
    MCF10A cell line
    FACL4 Antibody NBP2-16401

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