FAK [p Tyr397] Antibody

Novus Biologicals | Catalog # NBP3-12897

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all FAK Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr397 of human FAK. The exact sequence is proprietary.

Modification

p Tyr397

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for FAK [p Tyr397] Antibody

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500.
Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897]

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] - Untreated (-) and treated (+) HUVEC whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
FAK [p Tyr397] Antibody

Immunocytochemistry/ Immunofluorescence: FAK [p Tyr397] Antibody [NBP3-12897] -

Immunocytochemistry/ Immunofluorescence: FAK [p Tyr397] Antibody [NBP3-12897] - FAK antibody detects FAK protein at cell junction, nucleus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAK stained by FAK antibody (NBP3-12897) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
FAK [p Tyr397] Antibody

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FAK [p Tyr397] Antibody

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -

Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FAK [p Tyr397] Antibody

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -

Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FAK [p Tyr397] Antibody

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -

Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FAK [p Tyr397] Antibody

Western Blot: FAK [p Tyr397] Antibody [NBP3-12897] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr397) antibody (NBP3-12897) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for FAK [p Tyr397] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAK

FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Gene Symbol

PTK2

Additional FAK Products

Product Documents for FAK [p Tyr397] Antibody

Certificate of Analysis

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Product Specific Notices for FAK [p Tyr397] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail