FAM171B Antibody - BSA Free

Western Blot: FAM171B Antibody - BSA Free [NBP1-93847]

Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate

Western Blot: FAM171B Antibody - BSA Free [NBP1-93847] -

FAM171B promotes the malignant phenotype of bladder cancer cells. A The Chronos dependency score analysis of FAM171B in the 28 human bladder urothelial carcinoma cell lines in the CCLE database. The Chronos dependency score is based on data from a cell depletion assay. A lower Chronos score indicates a higher likelihood that the gene of interest is essential in a given cell line. B Protein levels of FAM171B in the knockdown and overexpression cell lines. C Representative colony formation images in different groups. D Colony formation analysis of T24 and MB49 cells after knockdown and overexpression of FAM171B. E Cell viability of T24 and MB49 after knockdown and overexpression of FAM171B. F Representative transwell images of T24 and MB49 cells after knockdown and overexpression of FAM171B. G Statistical results of the number of invasive cells in each group of T24 and MB49 cells. H Representative images of migration in T24 and MB49 cells after knockdown and overexpression of FAM171B. I Statistical results of the number of migrated cells in each group of T24 and MB49 cells Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37915048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FAM171B Antibody - BSA Free [NBP1-93847] -

FAM171B regulates CCL2 via HNRNPU and promotes TAM migration and infiltration in bladder cancer. A ELISA analysis of CCL2 secretion levels in MB49 cells with FAM171B overexpression or knockdown. B RIP results show that HNRNPU can immunoprecipitate with CCL2 precursor RNA. C qRT-PCR analysis of the relative RNA levels of precursor CCL2 and CCL2 in MB49 cells with HNRNPU knockdown or control. D ELISA analysis of CCL2 secretion levels in MB49 cells with HNRNPU knockdown or control. E Protein levels of FAM171B and HNRNPU in the MB49 cell lines in different groups. F ELISA analysis of CCL2 secretion levels in MB49 cells in different groups. G Analysis of migration of RAW264.7(mouse macrophages) toward MB49 cells in different groups. H Representative images of migration of RAW264.7(mouse macrophages) toward MB49 cells in co-culture chambers. I The pictures of tumors derived from mouse bladder cancer MB49 cell line. J IHC staining pictures of F4/80(macrophage marker) expression in different groups. K Representative images of flow cytometry used to evaluate F4/80 positive subpopulations. L The tumor weight in different groups. M The tumor volume in different groups. N The average body weight of mice in different groups. O Analysis of F4/80 positive population by IHC. P Analysis of F4/80 positive population by flow cytometry Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37915048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FAM171B Antibody - BSA Free [NBP1-93847] -

FAM171B interacts with vimentin and HNRNPU in bladder cancer cells. A Mass spectrometry analysis of interacting proteins enriched by immunoprecipitation. Immunoprecipitation is performed by Anti-DYKDDDDK beads on T24 cells transfected with FLAG-FAM171B or FLAG-control. B Analysis of positive and background proteins based on Mass Spectrometry scores. C The top 16 proteins with the highest scores based on MS results. D Protein interaction network map of proteins bound to FAM171B based on MS results. The protein interactions data is from STRING database. E The predicted protein structure image of FAM171B based on the Alpha Fold. F Immunofluorescence analysis images revealed the localization of FAM171B in T24 cells. G Colocalization of FAM171B and vimentin was visualized by confocal microscope in T24 cells. Cytoplasmic staining of FAM171B and vimentin was mostly merged together. H Colocalization of FAM171B and HNRNPU was visualized by confocal microscope in T24 cells. Nuclear staining of FAM171B and HNRNPU was mostly merged together. I ImageJ was used for colocation analysis of FAM171B and vimentin. Pearson correlation analysis showed that r > 0.5. Manders’ colocation coefficient showed that M1 > 0.5 and M2 > 0.5. J Computational docking model between FAM171B and vimentin. K ImageJ was used for colocation analysis of FAM171B and HNRNPU. Pearson correlation analysis showed that r > 0.5. Manders’ colocation coefficient showed that M1 > 0.5 and M2 > 0.5. L Computational docking model between FAM171B and HNRNPU Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37915048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: FAM171B Antibody - BSA Free [NBP1-93847] -

FAM171B interacts with vimentin and HNRNPU in bladder cancer cells. A Mass spectrometry analysis of interacting proteins enriched by immunoprecipitation. Immunoprecipitation is performed by Anti-DYKDDDDK beads on T24 cells transfected with FLAG-FAM171B or FLAG-control. B Analysis of positive and background proteins based on Mass Spectrometry scores. C The top 16 proteins with the highest scores based on MS results. D Protein interaction network map of proteins bound to FAM171B based on MS results. The protein interactions data is from STRING database. E The predicted protein structure image of FAM171B based on the Alpha Fold. F Immunofluorescence analysis images revealed the localization of FAM171B in T24 cells. G Colocalization of FAM171B and vimentin was visualized by confocal microscope in T24 cells. Cytoplasmic staining of FAM171B and vimentin was mostly merged together. H Colocalization of FAM171B and HNRNPU was visualized by confocal microscope in T24 cells. Nuclear staining of FAM171B and HNRNPU was mostly merged together. I ImageJ was used for colocation analysis of FAM171B and vimentin. Pearson correlation analysis showed that r > 0.5. Manders’ colocation coefficient showed that M1 > 0.5 and M2 > 0.5. J Computational docking model between FAM171B and vimentin. K ImageJ was used for colocation analysis of FAM171B and HNRNPU. Pearson correlation analysis showed that r > 0.5. Manders’ colocation coefficient showed that M1 > 0.5 and M2 > 0.5. L Computational docking model between FAM171B and HNRNPU Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37915048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: FAM171B Antibody - BSA Free [NBP1-93847] -

FAM171B expression is positively correlated with tumor progression and M2 TAM infiltration in BLCA patients. A Analysis of FAM171B expression in tumor tissue and paired normal tissue from TCGA dataset. B Representative IHC staining images of FAM171B in BLCA tissue array. C IHC score in different T stages from BLCA tissue array. D IHC score in different N stages from BLCA tissue array. E Relationship between FAM171B expression level and overall survival from TCGA dataset. F Relationship between FAM171B expression level and progression free survival from TCGA dataset. G Relationship between FAM171B expression level and disease specific survival from TCGA dataset. H Relationship between FAM171B expression level and disease-free survival from TCGA dataset. I Relationship between FAM171B expression level and overall survival from GEO dataset (GSE13507/GSE31684/GSE32894/GSE37815). J Analysis of FAM171B expression in different T stages from TCGA dataset. K Analysis of FAM171B expression in different N stages from TCGA dataset. L Analysis of FAM171B expression in different pathologic stages from TCGA dataset. M Lollipop plot demonstrating the correlation between FAM171B expression and different immune cell infiltration. N Heat map of the correlation between FAM171B expression and major monocyte/macrophage chemokines. O Immunoscore analysis of FAM171B and M1 macrophages in bladder cancer using QUANTISEQ algorithm. P Immunoscore analysis of FAM171B and M2 macrophages in bladder cancer using QUANTISEQ algorithm. Q Representative IHC staining images (FAM171B) and IF staining images (CD206, CD8 and alpha -SMA) in BLCA tissue array. R Proportion of immunofluorescence-positive cells (CD206, CD8 and alpha -SMA) in FAM171B high or low expression group from tissue array Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37915048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.