FAM60A Antibody - Azide and BSA Free

Knockdown Validated: FAM60A Antibody - Azide and BSA Free [H00058516-B02P] -

FAM60A knockdown prevents PDAC tumor growth and promotes gemcitabine sensitivity. (A) The efficiency of FAM60A knockdown by transfecting siRNAs was evaluated by qRT-PCR; n = 3. (B) CCK-8 experiments detected the efficiency of using siRNAs knockdown FAM60A on cell proliferation; n = 3. (C) Western blotting experiments verified the efficiency of knockdown FAM60A by shRNA. (D) Plate clonal formation experiments detected the effect of FAM60A stable knockdown on cell survival; n = 3. (E) Cell viability was measured in sh-FAM60A-NC, sh-FAM60A-1, and sh-FAM60A-2 cells treated with the 10 nM gemcitabine (Gem); n = 3. (F) Examples of bioluminescent pictures taken from luciferase-expressing Capan-1 cells that were orthotopically implanted into nude mice treated with 0.9% sodium chloride or gemcitabine (50 mg/kg). (G) Quantification of mice’s flux luminescence is conducted with IVIS. (H) Survival curves of mice implanted with sh-FAM60A-NC or sh-FAM60A-1 cells respectively treated with NaCl or gemcitabine and 0.9% NaCl (n = 5). ***P < 0.001, **P < 0.01, *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38314086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.