FAT4 Antibody [FITC]

Novus Biologicals | Catalog # NBP1-78381F

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all FAT4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7]

Localization

Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAT4 Antibody [FITC]

Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -

FAT4-Antibody-[FITC]-Knockout-Validated-NBP1-78381F-img0001.jpg

Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -

FAT4-Antibody-[FITC]-Knockout-Validated-NBP1-78381F-img0002.jpg
FAT4 Antibody [FITC]

Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] -

Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model(A) Nude mice xenografted (n=5) with BGC-823-FAT4-KO cells (left side) show increased tumor growth (size) than tumors in mice xenografted with control BGC-803-NC cells (right side). (B) BGC-823-FAT4-KO cell derived xenograft tumors show increased tumor volume than control BGC-823-KO-NC cells (*P<0.05). The data points represent mean tumor volume ± SD measured on the indicated days. (C) Representative images show IHC staining of BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors with antibodies against FAT4 & Ki-67. Scale bar, 100 μm. (D) Histogram plots show percent Ki-67 positive cells in BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors. As shown, the Ki-67 index is higher in the BGC-823-FAT4-KO derived tumors than BGC-823-KO-NC derived tumors. Data represent mean ± SD from 3 independent experiments. * denotes P < 0.05 compared to control. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.23702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FAT4 Antibody [FITC]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FAT4

FAT4 (FAT tumor suppressor homolog 4, also know as Fat-J, mFat4, and CDHF14) is a member of the Cadherin superfamily. Members of the Cadherin superfamily positively regulate planar cell polarity (PCP) in drosophila wings and play an important role in cell to cell adhesion interactions. FAT4 and other Cadherin family members have been used in systems medicine; especially in the fields of oncology and neurology (PMID: 16865240). FAT4 is widely expressed in most tissues, especially fetal and infant brain. It is also expressed in brain tumors and colorectal cancer. Furthermore, human breast tumor cell lines and primary tumors show a loss of FAT4 expression. Promoter methylation of a FAT4 allele can inactivate the expression of the FAT4 gene in deleted regions of the Hippo signaling pathway, the pathway that controls organ size (PMID: 19048595).

Alternate Names

cadherin family member 14, cadherin-related family member 11, CDHR11, FAT tumor suppressor homolog 4 (Drosophila), FAT-J, fat-like cadherin protein FAT-J, FLJ77878, hFat4, NBLA00548, putative protein product of Nbla00548

Gene Symbol

FAT4

Additional FAT4 Products

Product Documents for FAT4 Antibody [FITC]

Certificate of Analysis

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Download SDS

Product Specific Notices for FAT4 Antibody [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FAT4 Antibody [FITC]

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Protocols

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FAQs

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