Fatty Acid Synthase/FASN Antibody (3F2-1F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00002194-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Sandwich ELISA Capture, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F2-1F3

Format

Azide and BSA Free
View all Fatty Acid Synthase/FASN Primary Antibodies »

Product Specifications

Immunogen

FASN (AAH07909, 1 a.a. ~ 439 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASGLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Specificity

FASN - fatty acid synthase (3F2-1F3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Fatty Acid Synthase/FASN Antibody (3F2-1F3) - Azide and BSA Free

Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody (M01), clone 3F2-1F3.Lane 1: FASN transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of monoclonal antibody to FASN on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of monoclonal antibody to FASN on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 10 ug/ml.
Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Western Blot: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - FASN monoclonal antibody (M01), clone 3F2-1F3 Analysis of FASN expression in 293.
Immunoprecipitation: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Immunoprecipitation: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Immunoprecipitation: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Analysis of FASN transfected lysate using anti-FASN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FASN MaxPab rabbit polyclonal antibody.
Sandwich ELISA Capture: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Sandwich ELISA Capture: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01]

Sandwich ELISA Capture: Fatty Acid Synthase/FASN Antibody (3F2-1F3) [H00002194-M01] - Detection limit for recombinant GST tagged Fatty Acid Synthase/FASN is 0.1 ng/ml as a capture antibody.

Applications for Fatty Acid Synthase/FASN Antibody (3F2-1F3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fatty Acid Synthase/FASN

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Alternate Names

FASN, OA-519, SDR27X1

Gene Symbol

FASN

OMIM

600212 (Human)

Additional Fatty Acid Synthase/FASN Products

Product Documents for Fatty Acid Synthase/FASN Antibody (3F2-1F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fatty Acid Synthase/FASN Antibody (3F2-1F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fatty Acid Synthase/FASN Antibody (3F2-1F3) - Azide and BSA Free

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Protocols

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