Fc gamma RII/CD32 Antibody

Novus Biologicals | Catalog # NB100-1532

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Fc gamma RII/CD32 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus according to NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1.

Epitope

C-PDALEEPDDQNRI

Specificity

This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Fc gamma RII/CD32 Antibody

Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532]

Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532]

Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532]

Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532]

Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Staining (8ug/ml) of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.
Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532]

Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532]

Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
Fc gamma RII/CD32 Antibody

Immunocytochemistry/Immunofluorescence: Goat Polyclonal Fc gamma RII/CD32 Antibody [NB100-1532] -

Immunocytochemistry/Immunofluorescence: Goat Polyclonal Fc gamma RII/CD32 Antibody [NB100-1532] - Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Fc gamma RII/CD32 Antibody

Application
Recommended Usage

ELISA

1:32000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

6 - 8 ug/ml

Immunohistochemistry-Paraffin

6 - 8 ug/ml

Western Blot

0.3-1 ug/ml
Application Notes
WB: Approx 38 kDa band observed in Daudi lysates (calculated MW of 34.0 kDa according to NP_003992.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fc gamma RII/CD32

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Entrez Gene IDs

2213 (Human)

Gene Symbol

FCGR2B

UniProt

NP_003992.3 (Human)

Additional Fc gamma RII/CD32 Products

Product Documents for Fc gamma RII/CD32 Antibody

Certificate of Analysis

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Product Specific Notices for Fc gamma RII/CD32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fc gamma RII/CD32 Antibody

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Protocols

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