Fc gamma RIIIB/CD16b Antibody (BLR163J)

Novus Biologicals | Catalog # NBP3-14737

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR163J
View all Fc gamma RIIIB/CD16b Primary Antibodies »

Product Specifications

Immunogen

residues 21-201 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Fc gamma RIIIB/CD16b Antibody (BLR163J)

Western Blot: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737]

Western Blot: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737]

Western Blot: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737] - Whole cell lysate (50 ug) from HeLa, HEK293T, PBMCs, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Rabbit anti-CD16 recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Immunohistochemistry: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737]

Immunohistochemistry: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737]

Immunohistochemistry: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737] - Rabbit anti-CD16 recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737]

Immunohistochemistry: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737]

Immunohistochemistry: Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737] - Rabbit anti-CD16 recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.
Fc gamma RIIIB/CD16b Antibody (BLR163J)

Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737] -

Detection of human CD16 in FFPE CD16A transfectedHEKES cells (left) and CD16B transfected HEKES cells(right) by immunocytochemistry. Antibody: Rabbit antiCD16A recombinant monoclonal antibody [BLR163J] Secondary: HRP-conjugated goat antirabbit IgG Substrate: DAB.
Fc gamma RIIIB/CD16b Antibody (BLR163J)

Fc gamma RIIIB/CD16b Antibody (BLR163J) [NBP3-14737] -

Detection of human CD16 in FFPE renal cell carcinoma byimmunohistochemistry. Antibody: Rabbit anti-CD16Arecombinant monoclonal antibody [BLR163J] Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.

Applications for Fc gamma RIIIB/CD16b Antibody (BLR163J)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Western Blot

1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Fc gamma RIIIB/CD16b

CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Long Name

Fc gamma Receptor III

Alternate Names

CD16b, FCG3, FCGR3B, FcgRIIIB, FcR-10, FCRIIIB, IGFR3

Gene Symbol

FCGR3B

Additional Fc gamma RIIIB/CD16b Products

Product Documents for Fc gamma RIIIB/CD16b Antibody (BLR163J)

Certificate of Analysis

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Product Specific Notices for Fc gamma RIIIB/CD16b Antibody (BLR163J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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