Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PF12J-10A

Format

BSA Free
View all Feline Leukaemia Virus p27 Primary Antibodies »

Product Specifications

Immunogen

Made to Viral FELINE LEUKAEMIA VIRUS p27

Specificity

NB100-65688 recognizes feline leukaemia virus (FeLV) p27 core protein (p27). The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and diagnosis is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Feline Leukaemia Virus p27 Antibody (PF12J-10A) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1500
Application Notes
In WB detects a band of approximately 28kDa under reducing conditions. A strong band of 80kDa representing glycosylated FeLV gag gene product may also be present.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Feline Leukaemia Virus p27

The feline leukemia virus (FeLV) is the causative agent of the most important fatal infectious disease complex of domestic cats today. It is an RNA virus belonging to the family Retroviridae.

Alternate Names

feline leukemia provirus, Feline leukemia virus p27, Feline leukemia virus subgroup c receptor related protein 1, Feline sarcoma virus, flvcr protein, flvcr1, FPS, hflvcr, Oncogene fes

UniProt

O89811 (Virus)

Additional Feline Leukaemia Virus p27 Products

Product Documents for Feline Leukaemia Virus p27 Antibody (PF12J-10A) - BSA Free

Certificate of Analysis

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Product Specific Notices for Feline Leukaemia Virus p27 Antibody (PF12J-10A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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