Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FEN-1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FEN-1 Antibody - BSA Free

Western Blot: FEN-1 Antibody [NB100-321]

Western Blot: FEN-1 Antibody [NB100-321]

Western Blot: FEN-1 Antibody [NB100-321] - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]

Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321]

Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321] - FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Fen1 (NB100-321) used at a dilution of 1:1000 (1ug/ml). Detection: DAB
Immunoprecipitation: FEN-1 Antibody [NB100-321]

Immunoprecipitation: FEN-1 Antibody [NB100-321]

Immunoprecipitation: FEN-1 Antibody [NB100-321] - Detection of human Fen1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 (lot NB100-321-2) used for IP at 6 ug per reaction. Fen1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-321-1). For blotting immunoprecipitated Fen1, NB100-321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for FEN-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200- 1:1000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. 16-18 hour incubation is recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FEN-1

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Long Name

Flap Endonuclease 1

Alternate Names

FEN1

Entrez Gene IDs

2237 (Human)

Gene Symbol

FEN1

UniProt

NP_004102.1 (Human)

Additional FEN-1 Products

Product Documents for FEN-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FEN-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

Citations for FEN-1 Antibody - BSA Free

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Protocols

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