Ferritin Light Chain Antibody (FTL/1386)

Novus Biologicals | Catalog # NBP2-53295

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # FTL/1386
View all Ferritin Light Chain Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 38-165) of human Ferritin Light Chain protein (exact sequence is proprietary) (Uniprot: P02792)

Localization

Cytoplasmic

Marker

Node-Negative Breast Tumor Prognostic Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54574)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ferritin Light Chain Antibody (FTL/1386)

Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Western Blot Analysis of human HeLa cell lysate using Ferritin Light Chain Antibody (FTL/1386).
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with Ferritin Light Chain Antibody (FTL/1386).
Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Western Blot: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Western Blot of A431, HeLa, Liver and Testis lysate using Ferritin Light Chain Antibody (FTL/1386).
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1386).
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1386).
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded human kidney stained with Biotinylated Ferritin Light Chain Mouse Monoclonal Antibody (FTL/1386).
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with Ferritin Light Chain Mouse Monoclonal Antibody (FTL/1386).
Protein Array: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Protein Array: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295]

Protein Array: Ferritin Light Chain Antibody (FTL/1386) [NBP2-53295] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ferritin Light Chain Antibody (FTL/1386) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Ferritin Light Chain Antibody (FTL/1386)

Application
Recommended Usage

Immunohistochemistry

0.25-0.5 ug/ml

Immunohistochemistry-Paraffin

0.1 - 0.2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistology (Formalin-fixed): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ferritin Light Chain

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Alternate Names

Ferritin L subunit, ferritin L-chain, ferritin light chain, ferritin light polypeptide-like 3, ferritin, light polypeptide, MGC71996, NBIA3

Gene Symbol

FTL

UniProt

P02792 (Human)

Additional Ferritin Light Chain Products

Product Documents for Ferritin Light Chain Antibody (FTL/1386)

Certificate of Analysis

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Product Specific Notices for Ferritin Light Chain Antibody (FTL/1386)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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