Fetal Bovine Serum - Premium

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Catalog # Availability Size / Price Qty
S11110
S11150
S11195
Fetal Bovine Serum - Premium
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Fetal Bovine Serum - Premium Summary

Fetal Bovine Serum – Premium is a low-endotoxin, low-hemoglobin FBS that offers excellent value for basic cell culture as well as for specific research applications. Premium grade FBS is most widely used due to its unique combination of quality and value. Fetal Bovine Serum – Premium demonstrates excellent performance with a broad spectrum of cell types.

Key Benefits

• Most widely used FBS from R&D Systems
• Excellent value and performance for general cell culture applications
• High lot-to-lot consistency
• Manufactured from USA-origin or USDA-approved
• Excellent cell growth characteristics, low endotoxin, and low hemoglobin levels
• Manufactured in our ISO 9001:2015 certified facility
• Formerly sold under the Atlanta Biologicals brand

Why Use Fetal Bovine Serum - Premium?

Fetal Bovine Serum, also known as Fetal Calf Serum (FCS), is a critical supplement added to cell culture medium to maintain cell health and promote expansion. Different grades of FBS are available from R&D Systems that address the value and premium needs of research.

All lots of Fetal Bovine Serum – Premium are sterility, virus, and performance tested, and include a biochemical profile. Lots are manufactured from USA origin or USDA-approved origin material following stringent manufacturing and quality control procedures. All lots of FBS - Premium are manufactured in our ISO 9001:2015 certified facility.

Fetal Bovine Serum and other serum products should be stored and handled correctly to assure long-term stability and to preserve growth performance consistency throughout its shelf-life. In addition, heat inactivation of serum is frequently desired to inactivate complement within the serum. Below are protocol links for serum storage/handling and heat inactivation.

Protocol for Fetal Bovine Serum Storage, Thawing, and Freezing.
Protocol for Heat Inactivation of Serum Products.

Collection, Processing, and Testing of R&D Systems FBS

Collection and Processing - R&D Systems FBS is manufactured under a process that meets USDA requirements for animal products and ensures the product is traceable back to the date and location of collection. Our FBS manufacturing adheres to closed-system collection, processing in positive pressure, HEPA-filtered clean rooms, and triple 0.1 um filtration to remove contaminants. Strict control of the process from collection through processing enables us to provide a stable, consistent performing, and traceable supply of quality serum throughout global fluctuations in serum availability and regulatory requirements.

Virus Testing - Fetal Bovine Serum – Premium undergoes testing for adventitious viruses, such as: Bovine Viral Diarrhea Virus (BVDV), Infectious Bovine Rhinotracheitis Virus (IBRV), Parainfluenza-3 Virus (PI-3V), Bluetongue Virus (BTV), Bovine Respiratory Syncytial Virus (BRSV), Bovine Parvovirus (BPV), Bovine Adenovirus, Type 3 (BAV-3), Bovine Adenovirus, Type 5 (BAV-5), Reo virus, and Rabies virus.

Biochemical Testing - R&D Systems FBS undergo biochemical profiling including total protein, albumin, globulin, IgG, total bilirubin, glucose, iron, cholesterol, triglycerides, sodium and potassium. View the product insert for a complete list of biochemical profiling components, which may vary between grades.

Chemical Analysis - Each lot of FBS is tested for osmolality, pH, hemoglobin, endotoxin (LAL method) and microbiological contamination (using methods recommended by the U.S. Pharmacopeia).

Specifications

Osmolality
280 -335 mOsm
Purification
Triple 0.1 μm filtered
Sterility Testing
Pass
Testing Cell Culture
Pass
Testing Biochemical Test Panel
Extended
Viral Testing
Standard
Testing Additional
pH - 6.8-7.8

Endotoxin - ≤ 50.0 EU/mL by Limulus Amebocyte Lysate (LAL) Assay

Hemoglobin - 25 mg/dL
Shipping Conditions
On Dry Ice
Storage
-5 to -20 °C
Applications
Cell Culture
Species
Bovine

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Fetal Bovine Serum - Premium

Premium Fetal Bovine Serum Displays Lot-to-Lot Consistency. Quality control data for over 80 lots of Premium FBS shows high levels of performance consistency. (A) Plating efficiency of cells cultured in media containing 5% or 10% Premium FBS, relative to control lots of FBS. (B) Premium FBS was tested for its ability to support hybridoma cloning as well as the healthy expansion of adherent and suspension cell culture lines. Lots of Premium FBS show consistent and high percentage cloning efficiency and cell viability.

Growth of Mesenchymal Stem Cells using Premium FBS. Human mesenchymal stem cells (MSCs) were cultured for 4 passages in media supplemented with Premium FBS or an alternate FBS supplier (Supplier #1). MSCs were analyzed by flow cytometry using PE-conjugated CD90 (R&D Systems, Catalog # FAB2067P) and APC-conjugated CD45 (R&D Systems, Catalog # FAB1430A). (A) Percentage of CD90+ MSCs following four passages in culture. (B) Representative flow cytometry data of CD90+ human MSCs cultured for 4 passages in Premium Grade FBS.

Reagents Provided

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