Human CD90/Thy1 PE‑conjugated Antibody

Name: Human CD90/Thy1 PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB2067P
Price: 329 USD
Availability: InStock
Rating: 5 (1 reviews)

R&D Systems | Catalog # FAB2067P

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Citations (36)

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Equine

Applications

Validated:

Knockout Validated, Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG_2A Clone # Thy-1A1

View all CD90/Thy1 Primary Antibodies »

Product Specifications

Immunogen

Human CD90/Thy1

Specificity

Detects human CD90/Thy1 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human CD90/Thy1 PE‑conjugated Antibody

Detection of CD90/Thy1 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CD90/Thy1 PE-conjugated Monoclonal Antibody (Catalog # FAB2067P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

CD90/Thy1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

CD90/Thy1 knockout Jurkat human cell line was stained with Mouse Anti-Human CD90/Thy1 Monoclonal Antibody (Catalog # FAB2067P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). No staining in the CD90/Thy1 knockout Jurkat cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD90/Thy1 PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line

Knockout Validated

CD90/Thy1 is specifically detected in Jurkat human parental cell line but is not detectable in CD90/Thy1 knockout Jurkat cell line.

Reviewed Applications

Read 1 review rated 5 using FAB2067P in the following applications:

Flow Cytometry (1 Review)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD90/Thy1

CD90, also known as Thy1, is a 25-35 kDa GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, neurons, fibroblasts, greater alveolar type II progenitors, and mesangial cells. It binds to Integrin alpha V beta 3, and participates in bidirectional signaling, with CD90 itself impacting Src-family kinases. Notably, CD90 has been identified as a cell-surface mediator of cytomegalovirus infection. Mature human CD90 shares 62% amino acid identity with mouse CD90.