FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation. A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells.
FGF-4 Antibody (3A12G9) - BSA Free
Novus Biologicals | Catalog # NBP2-37374
Loading...
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3A12G9
Format
BSA Free
Loading...
Product Specifications
Immunogen
Purified recombinant fragment of humanFGF-4 (AA: 62-123) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FGF-4 Antibody (3A12G9) - BSA Free
Western Blot: FGF-4 Antibody (3A12G9)BSA Free [NBP2-37374]
Western Blot: FGF-4 Antibody (3A12G9) [NBP2-37374] - Western blot analysis using FGF4 mAb against HEK293 (1) and FGF4 (AA: 62-123)-hIgGFc transfected HEK293 (2) cell lysate.Western Blot: FGF-4 Antibody (3A12G9)BSA Free [NBP2-37374]
Western Blot: FGF-4 Antibody (3A12G9) [NBP2-37374] - Western blot analysis using FGF4 mAb against human FGF4 recombinant protein. (Expected MW is 32.6 kDa)ELISA: FGF-4 Antibody (3A12G9) - BSA Free [NBP2-37374]
ELISA: FGF-4 Antibody (3A12G9) [NBP2-37374] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Applications for FGF-4 Antibody (3A12G9) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FGF-4
Long Name
Fibroblast Growth Factor 4
Alternate Names
FGF4, HBGF-4, HST-1, KFGF, KS3
Gene Symbol
FGF4
Additional FGF-4 Products
Product Documents for FGF-4 Antibody (3A12G9) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FGF-4 Antibody (3A12G9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for FGF-4 Antibody (3A12G9) - BSA Free
There are currently no reviews for this product. Be the first to review FGF-4 Antibody (3A12G9) - BSA Free and earn rewards!
Have you used FGF-4 Antibody (3A12G9) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars