FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation. A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells.
FGF-4 Antibody (678430) [DyLight 405]
Novus Biologicals | Catalog # FAB58461E
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 678430
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Product Specifications
Immunogen
E. coli-derived recombinant mouse FGF-4
Ala30-Leu202
Accession # P11403
Ala30-Leu202
Accession # P11403
Specificity
Detects mouse FGF-4 in direct ELISAs and Western blots. In Western blots, 100% cross-reactivty with
recombinant human (rh) FGF-4 and rhFGF-7 is observed. No cross-reactivity with
rhFGF-3, -5, -9, -17, recombinant mouse FGF-6, -8b, -8c, or -10 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for FGF-4 Antibody (678430) [DyLight 405]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FGF-4
Long Name
Fibroblast Growth Factor 4
Alternate Names
FGF4, HBGF-4, HST-1, KFGF, KS3
Gene Symbol
FGF4
Additional FGF-4 Products
Product Documents for FGF-4 Antibody (678430) [DyLight 405]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FGF-4 Antibody (678430) [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars