Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 678430
View all FGF-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse FGF-4
Ala30-Leu202
Accession # P11403

Specificity

Detects mouse FGF-4 in direct ELISAs and Western blots. In Western blots, 100% cross-reactivty with recombinant human (rh) FGF-4 and rhFGF-7 is observed. No cross-reactivity with rhFGF-3, -5, -9, -17, recombinant mouse FGF-6, -8b, -8c, or -10 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse FGF‑4 Antibody

Detection of Mouse FGF-4 antibody by Western Blot.

Detection of Mouse FGF‑4 by Western Blot.

Western blot shows lysates of 4T1 mouse breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse FGF-4 Monoclonal Antibody (Catalog # MAB58461) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FGF-4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse FGF‑4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 4T1 mouse breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-4

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The mouse FGF-4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF‑4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3‑5). Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6‑9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10‑12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

References

  1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
  3. Niswander, L. and G.R. Martin (1992) Development 114:755.
  4. Feldman, B. et al. (1995) Science 267:246.
  5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
  6. Sun, X. et al. (2002) Nature 418:501.
  7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
  8. Yu, K and D.M. Ornitz (2008) Development 135:483.
  9. Mariani, F.V. et al. (2008) Nature 453:401.
  10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
  11. Kunath, T. et al. (2007) Development 134:2895.
  12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
  13. Hajitou, A. et al. (1998) Oncogene 17:2059.
  14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

Long Name

Fibroblast Growth Factor 4

Alternate Names

FGF4, HBGF-4, HST-1, KFGF, KS3

Entrez Gene IDs

2249 (Human); 14175 (Mouse); 116499 (Rat)

Gene Symbol

FGF4

UniProt

P11403

Additional FGF-4 Products

Product Documents for Mouse FGF‑4 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse FGF‑4 Antibody

For research use only

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