Detects mouse FGF-4 in direct ELISAs and Western blots. In Western blots, 100% cross-reactivty with
recombinant human (rh) FGF-4 and rhFGF-7 is observed. No cross-reactivity with
rhFGF-3, -5, -9, -17, recombinant mouse FGF-6, -8b, -8c, or -10 is observed.
Monoclonal Rat IgG2A Clone # 678430
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse FGF-4 Ala30-Leu202 Accession # P11403
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse FGF‑4 by Western Blot. Western blot shows lysates of 4T1 mouse breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse FGF‑4 Monoclonal Antibody (Catalog # MAB58461) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FGF‑4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s
sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of
the FGF family (1, 2). The mouse FGF-4 cDNA encodes 202 amino acids
(aa) with a 29 aa signal sequence and a 173 aa mature protein with an
FGF homology domain that contains a heparin binding region near the
C-terminus (2). Mature mouse FGF-4 shares 87%, 90%, 87% and 85% aa
identity with human, rat, canine and bovine FGF‑4, respectively. Human
FGF-4 has been shown to exhibit cross species activity. Expression of
FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and
temporally regulated during embryonic development (1, 3). Its expression
in the trophoblast inner cell mass promotes expression of FGF R2, and
is required for maintenance of the trophectoderm and primitive endoderm
(3‑5). Later in development, FGF-4 works together with FGF-8 to mediate
the activities of the apical ectodermal ridge, which direct the
outgrowth and patterning of vertebrate limbs (3, 6‑9). FGF-4 is proposed
to play a physiologically relevant role in human embryonic stem cell
self-renewal. It promotes stem cell proliferation, but may also aid
differentiation depending on context and concentration, and is often
included in embryonic stem cell media in vitro (10‑12). A
C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and
promotes differentiation is endogenously expressed in human embryonic
stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).
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Fibroblast Growth Factor 4
Entrez Gene IDs:
2249 (Human); 14175 (Mouse); 116499 (Rat)
FGF4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST
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