FGFR1 Antibody (M17A3) - BSA Free

Novus Biologicals | Catalog # NB100-2079

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # M17A3

Format

BSA Free
View all FGFR1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ectodomain of FGF R1 expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

Epitope is undetermined but presumed to be near junction of the common FGF R1 NH2-terminus and NH2-terminus of Ig loop II.

Localization

Cell Membrane

Specificity

Reacts with beta isoform of FRFR1. Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculoviral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: under investigation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for FGFR1 Antibody (M17A3) - BSA Free

Western Blot: FGFR1 Antibody (M17A3)BSA Free [NB100-2079]

Western Blot: FGFR1 Antibody (M17A3)BSA Free [NB100-2079]

Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]

Immunohistochemistry-Paraffin: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]

Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml concentration. The keratinocytes of the epidermal layer showed a very strong membrane-cytoplasmic with relatively weak nuclear staining for FGF R1 Antibody. The stratum basale layer of keratinocytes depicted more of membrane-cytoplasmic staining for this protein.
Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]

Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]

Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.
Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]

Flow Cytometry: FGFR1 Antibody (M17A3) - BSA Free [NB100-2079]

Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.

Applications for FGFR1 Antibody (M17A3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500-1:1000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

2-4 ug/ml
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF R1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGF R1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGF R1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGF R1 result in Pfeiffer syndrome associated with craniosynostosis. FGF R1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGF R1 has been shown to interact with N-cadherin and NCAM.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human)

Gene Symbol

FGFR1

UniProt

P11362

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M17A3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FGFR1 Antibody (M17A3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FGFR1 Antibody (M17A3) - BSA Free

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Protocols

View specific protocols for FGFR1 Antibody (M17A3) - BSA Free (NB100-2079):

FGFR1 Antibody (M17A3):
1. Deparaffinize the tissue sections by immersing the slides in Xylene with two changes for 10 min each. Sections should not get dried at any stage from this point.
2. Rehydrate the tissue sections by immersing the slides in decreasing grades of ethanol as follows:
a. Immerse in 100% ethanol with 2 changes for 5 minutes each
b. Immerse in 95% ethanol with 2 changes for 5 minutes each
c. Immerse in 90% ethanol for 5 minutes
d. Immerse in 70% ethanol for 5 minutes
e. Immerse in 50% ethanol for 5 minutes
f. Immerse in distilled water for 5 minutes
3. Antigen Retrieval (Microwave Method):
a. Immerse the slides in a microwave compatible tray containing 10 mM Sodium Citrate buffer (pH 6.0) with 0.05% Tween 20.
b. Boil the slides and maintain the sub-boiling temperature for 5 minutes in the microwave. Thereafter, take out the tray very carefully and cool it at room temperature (RT) for about 30 minutes.
c. Wash the slides 3 times, 3 minutes each by immersing them in TBST (Tris Buffered Saline having 0.05% Tween 20).
4. Quenching of Endogenous Peroxidase:
a. Incubate the slides in 3% hydrogen peroxide prepared in methanol for 15 minutes (at RT, in dark conditions).
b. Wash the slides in TBST 3 times, 3 minutes each.
5. Protein Blocking:
a. Incubate the sections with background sniper solution at RT for 15 minutes (Biocare Medicals, USA).
b. Wash the sections 3 times, 3 min each by immersing the slides in TBST.
6. Primary Antibody:
a. Dilute the primary antibody at 5ug/ml concentration using PBS as a diluent.
b. Incubate the sections with diluted primary antibody for 90 minutes at RT in a humidified chamber.
c. Thereafter, wash the slides 4 times, 5 minutes each with TBST.
7. Probe (Secondary Reagent):
a. Incubate with MACH 1 Mouse probe for 15 minutes at RT.
b. Incubate for 30 min at room temperature with HRP-Polymer (Biocare Medical, USA).
c. Wash the slides with TBST 4 times, 5 minutes each
8. Chromogen:
a. Mix 32ul of DAB Chromogen with 1 ml of DAB substrate buffer (Biocare Medical, USA).
a. Apply 200ul DAB mixture/section and incubate at RT in dark conditions (few seconds - 5 minutes).
b. As soon as an appropriate color develops, rinse the slides with deionized water (2-3 brief rinses).
9. Counter stain:
a. Counter stain with Hematoxylin for 30 seconds (Vector Labs, USA).
b. Wash in deionized water for 1-2 minutes to clear the extra stain.
c. Incubate the slides in bluing solution or Scott's water twice for 2 minutes each time.
10. Dehydrate the sections in increasing grades of alcohols:
a. 50% alcohol for 1 minute
b. 70% for 1 minute
c. 90% for 1 minute
d. 95% for 1 minute
e. 100% for 1 minute
f. Xylene with 2 changes for 2 minutes each
11. Mount with DPX mount and cover-slip glass (Fisher Scientific, USA), carefully not allowing any air bubbles to enter.

NOTE:- This protocol is provided as a reference tool only. Depending upon the type of tissues /tissue processing and reagents employed, the end user will need to optimize the final conditions for achieving an expected staining.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FGFR1 Antibody (M17A3) - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Do you have any information on these questions regarding the flow picture on the product's datasheet? 1) Is the epitope extracellular? 2) Were the cells permeabilized before staining? 3) Is there any literature about this antibody and flow cytometry?

    A: In answer to your questions: 1) The epitope is undetermined but presumed to be near junction of the common FGFr1 NH2-terminus and NH2-terminus of Ig loop II. 2) The immunogen was made to the extracellular domain, so permeabilization was not performed. 3) We unfortunately do not have any publications with this particular antibody.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: Do you have any information on these questions regarding the flow picture on the product's datasheet? 1) Is the epitope extracellular? 2) Were the cells permeabilized before staining? 3) Is there any literature about this antibody and flow cytometry?

    A: In answer to your questions: 1) The epitope is undetermined but presumed to be near junction of the common FGFr1 NH2-terminus and NH2-terminus of Ig loop II. 2) The immunogen was made to the extracellular domain, so permeabilization was not performed. 3) We unfortunately do not have any publications with this particular antibody.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: Do you have any information on these questions regarding the flow picture on the product's datasheet? 1) Is the epitope extracellular? 2) Were the cells permeabilized before staining? 3) Is there any literature about this antibody and flow cytometry?

    A: In answer to your questions: 1) The epitope is undetermined but presumed to be near junction of the common FGFr1 NH2-terminus and NH2-terminus of Ig loop II. 2) The immunogen was made to the extracellular domain, so permeabilization was not performed. 3) We unfortunately do not have any publications with this particular antibody.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

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