FGFR1 Antibody (M17D10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80733

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M17D10

Format

Azide and BSA Free
View all FGFR1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ectodomain of FGFr1b expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

Epitope is within the sequence pro179 to lys195 in Ig loop II.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 8575296). Not yet tested in other species.

Localization

Cell Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FGFR1 Antibody (M17D10) - Azide and BSA Free

Western Blot: FGFR1 Antibody (M17D10)Azide and BSA Free [NBP2-80733]

Western Blot: FGFR1 Antibody (M17D10)Azide and BSA Free [NBP2-80733]

Western Blot: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody. Image from the standard format of this antibody.
Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunocytochemistry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein. Image from the standard forma
Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 prot
Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - FGFR1 (M17D10) antibody was tested in NTERA2 cells at 1:100 with DyLight 488 (Green) using a 40X objective. Actin and nuclei were counterstained with Phalloidin 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Immunohistochemistry-Paraffin: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer showed a very strong membrane-cytoplasmic staining with relatively weak nuclear immunopositivity for for th
Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733]

Flow Cytometry: FGFR1 Antibody (M17D10) - Azide and BSA Free [NBP2-80733] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown. Image from the standard format of this antibody.

Applications for FGFR1 Antibody (M17D10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/ml

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/ml
Application Notes
This antibody is CyTOF ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M17D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FGFR1 Antibody (M17D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FGFR1 Antibody (M17D10) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review FGFR1 Antibody (M17D10) - Azide and BSA Free and earn rewards!

Have you used FGFR1 Antibody (M17D10) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FGFR1 Antibody (M17D10) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies