FGFR1 Antibody (M19B2) - BSA Free

Novus Biologicals | Catalog # NB600-1287

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Rabbit

Cited:

Human, Canine, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M19B2

Format

BSA Free
View all FGFR1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ectodomain of FGFR1b expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

Epitope is within the NH2-terminus of Ig loop II sequence lys145 and glu159 between the acidic box and heparin-binding sequence of FGFR1.

Reactivity Notes

Use in Canine reported in scientific literature (PMID:34998203) Rabbit reactivity reported in scientific literature (PMID: 31755222)..

Localization

Cell Membrane

Specificity

Reacts with both alpha and beta isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FGFR1 Antibody (M19B2) - BSA Free

Western Blot: FGFR1 Antibody (M19B2)BSA Free [NB600-1287]

Western Blot: FGFR1 Antibody (M19B2)BSA Free [NB600-1287]

Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Flow Cytometry: FGFR1 Antibody (M19B2) - BSA Free [NB600-1287]

Flow Cytometry: FGFR1 Antibody (M19B2) - BSA Free [NB600-1287]

Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.
Flow Cytometry: FGFR1 Antibody (M19B2) - BSA Free [NB600-1287]

Flow Cytometry: FGFR1 Antibody (M19B2) - BSA Free [NB600-1287]

Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ug/1x10^6 cells was used.

Applications for FGFR1 Antibody (M19B2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 33637089)

Immunohistochemistry

reported in scientific literature (PMID 25182532)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 34998203)

Immunoprecipitation

1:10-1:500

Western Blot

1-2 ug/ml
Application Notes
This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NB600-1287 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 79114 (Rat)

Gene Symbol

FGFR1

UniProt

P11362, Q04589 (Rat)

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M19B2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FGFR1 Antibody (M19B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FGFR1 Antibody (M19B2) - BSA Free

Customer Reviews for FGFR1 Antibody (M19B2) - BSA Free (1)

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  • Name: Lisa Renshaw
    Application: Flow Cytometry
    Sample Tested: transfected cell line
    Species: Other
    Verified Customer | Posted 12/16/2010

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Protocols

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FAQs for FGFR1 Antibody (M19B2) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

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