FGFR1 Antibody (M2F12) - BSA Free

Novus Biologicals | Catalog # NB120-829

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M2F12

Format

BSA Free
View all FGFR1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.

Specificity

Reacts with alpha isoform of FRFR1. Reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for FGFR1 Antibody (M2F12) - BSA Free

Western Blot: FGFR1 Antibody (M2F12)BSA Free [NB120-829]

Western Blot: FGFR1 Antibody (M2F12)BSA Free [NB120-829]

Western Blot: FGF R1 Antibody (M2F12) [NB120-829] - Total protein from Ntera-2 was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/ml of anti-FGFR1. FGFR1 protein was detected at approximately 150 kDa using an anti-mouse HRP secondary antibody.
Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Immunocytochemistry/Immunofluorescence: FGF R1 Antibody (M2F12) [NB120-829] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with FGR1 (M2F12) (NB120-829) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in MCF-7 cells (1 x 10^6 cells/ml) stained with FGF r1 antibody (NB120-829, red peaks) at a 1:1000 dilution. Detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.
Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in HEK293 cells (2 x10^6) stained with FGF R1 antibody (NB120-829, red) at a 1:1000 dilution and detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.

Applications for FGFR1 Antibody (M2F12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

reported in scientific literature (PMID 8168112)

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/mL
Application Notes
Western blotting: 1 ug/mL recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1. Immunoprecipitation: Native, nuclear, recombinant, and ligand-labeled FGFr1. Customers have reported difficulties using this antibody in immunocytochemistry staining (see review). This antibody does not appear to work in IHC paraffin embedded sections.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse); 79114 (Rat)

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M2F12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FGFR1 Antibody (M2F12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FGFR1 Antibody (M2F12) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FGFR1 Antibody (M2F12) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

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