FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-70731
![Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Western-Blot-NBP2-70731-img0002.jpg "Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5C5
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human FGFR2(NP_000132) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]
Western Blot: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2.![Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70731-img0005.jpg "Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunocytochemistry/Immunofluorescence: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2.![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0009.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of paraffin-embedded Human breast tissue using anti-FGFR2 mouse monoclonal antibody.![Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70731-img0004.jpg "Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FGFR2 antibody, and then analyzed by flow cytometry.![Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Western-Blot-NBP2-70731-img0001.jpg "Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]")
Western Blot: FGFR2 Antibody (OTI5C5)Azide and BSA Free [NBP2-70731]
Western Blot: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Analysis of FGF R2 in HEK293T cells (left, control) and HEK293T cells overexpressing FGF R2 (right) using anti-FGF R2 antibody. Image from verified customer review.![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0006.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody.![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0007.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FGFR2 mouse monoclonal antibody.![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0008.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FGFR2 mouse monoclonal antibody.![Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70731-img0010.jpg "Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Immunohistochemistry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Staining of paraffin-embedded Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody.![Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]](https://resources.rndsystems.com/images/products/FGFR2-Antibody-OTI5C5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70731-img0003.jpg "Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]")
Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731]
Flow Cytometry: FGFR2 Antibody (OTI5C5) - Azide and BSA Free [NBP2-70731] - Analysis of Hela cells, using anti-FGFR2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:200 - 1:1000
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: FGFR2
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Gene Symbol
FGFR2
Additional FGFR2 Products
Product Documents for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FGFR2 Antibody (OTI5C5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
