FGFR2 Antibody

Novus Biologicals | Catalog # NB200-642

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all FGFR2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: KLPQYPHIGSVKT conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 809-821 of the cytoplasmic region of Human FGFR2.

Localization

Cytoplasmic

Specificity

FGFR-2. No reaction with human FGFR-1 and FGFR-3 is detected.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FGFR2 Antibody (NB200-642) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FGFR2 Antibody

Western Blot: FGFR2 Antibody [NB200-642]

Western Blot: FGFR2 Antibody [NB200-642]

Western Blot: FGFR2 Antibody [NB200-642] - Whole extract of HEK-293T cells expressing FGFR-2 were separated on SDS-PAGE and probed with Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit. The antibody was developed using 1:10,000 Goat Anti-Rabbit IgG-Peroxidase.Lanes:1. 0.5 ug/mL antibody2. 0.25 ug/mL antibody3. 0.5 ug/mL antibody untransfected 293T cells4. Negative Control
Immunohistochemistry: FGFR2 Antibody [NB200-642]

Immunohistochemistry: FGFR2 Antibody [NB200-642]

Immunohistochemistry: FGFR2 Antibody [NB200-642] - Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 2 ug/mL Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit followed by 20 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 20 ug/mL ExtrAvidin-Peroxidase.
Western Blot: FGFR2 Antibody [NB200-642]

Western Blot: FGFR2 Antibody [NB200-642]

Western Blot: FGFR2 Antibody [NB200-642] - Antibody recognition: Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in Rabbit is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Fibroblast Growth Factor Receptor-2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with 1:10,000 Goat Anti-Rabbit IgG-peroxidase and a chemiluminescent substrate.Antibody dilution: 0.5 ug/mL.

Applications for FGFR2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

2-4 ug/ml

Western Blot

0.25-0.5 ug/ml
Application Notes
Although not tested this antibody may be useful in Immunohistochemistry-Frozen.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR2

Fibroblast growth factors (FGFs) are members of a large family of structurally related heparin binding polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation for a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. They have also been implicated in pathological conditions such as tumorigenesis and metastasis. The FGF family consists of at least seventeen members designated FGF1 through FGF17. To date, four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg, cek-1], FGFR2 [bek, cek-3], FGFR3 [cek-2] and FGFR4. Multiple additional variants (isoforms) arising by alternative splicing have been reported. Soluble, secreted8 or possibly cleaved9 forms of FGFR1 and FGFR2 have also been found in body fluids or were artificially constructed. FGFRs are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150 kD proteins that are constructed of an extracellular ligand binding region with either two or typically three immunoglobulin (Ig)-like domains and an eight amino acid 'acidic box', a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3.

FGFRs exhibit overlapping recognition and redundant specificity. One receptor type may bind several of the FGFs with a similar affinity. Also one FGF type may bind similarly to several distinct receptors. This accounts for the rather identical effects of different FGF ligands on common cell types. FGFs binding to cellular FGFRs depends on, or is markedly facilitated by the low-affinity interaction of FGFs with the polysaccharide component of cell surface or extracellular matrix heparan sulfate proteoglycans (HSPG). For example, perlecan, a basement membrane HSPG, promotes high affinity binding of FGF2 in vitro and angiogenesis in vivo. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ and are dictated by the intracellular domain. At the mRNA level, FGFR- 2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It is widely expressed in many adult human and animal tissues. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells. Crouzon as well as other craniosynostosis syndromes (e.g. Pfeiffer's, Apert's, Jackson-Weiss'), disorders of human skeletal development, have been shown to be the result of mutations in the extracellular domain of FGFR2.

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Entrez Gene IDs

2263 (Human)

Gene Symbol

FGFR2

UniProt

P21802

Additional FGFR2 Products

Product Documents for FGFR2 Antibody

Certificate of Analysis

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Product Specific Notices for FGFR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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