FAP (also known as seprase) is a transmembrane serine protease, and a soluble and enzymatically active form of FAP known as antiplasmin-cleaving enzyme (APCE) circulates in human plasma. FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis. It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV/CD26, which is specific for N-terminal Xaa-Pro sequences. FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion. In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity.
Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
Novus Biologicals | Catalog # NBP3-14728
Recombinant Monoclonal Antibody
Loading...
Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, COMET
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR150J
Loading...
Product Specifications
Immunogen
residues 26-760 (ECD)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
Detection of FAP in Human Melanoma via seqIF™ staining on COMET™
FAP was detected in immersion fixed paraffin-embedded sections of human Melanoma using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm of fibroblasts. Protocol available in COMET™ Panel Builder.
Detection of FAP in Human Colon Cancer via seqIF™ staining on COMET™
FAP was detected in immersion fixed paraffin-embedded sections of human Colon Cancer using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm of fibroblasts. Protocol available in COMET™ Panel Builder.
Detection of FAP in Human Pancreas via seqIF™ staining on COMET™
FAP was detected in immersion fixed paraffin-embedded sections of human Pancreas using Rabbit Anti-Human FAP, Monoclonal Antibody (Catalog# NBP3-14728) at 1:100 dilution at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of pancreatic islet cells. Protocol available in COMET™ Panel Builder.![Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) [NBP3-14728]](https://resources.rndsystems.com/images/products/Fibroblast-Activation-Protein-alpha-FAP-Antibody-Immunoprecipitation-NBP3-14728-img0001.jpg "Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) [NBP3-14728]")
Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) [NBP3-14728]
Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Whole cell lysate (500 ug per IP reaction; 5% of IPloaded) from IMR-90 cells. Antibody: Rabbit anti-FAPrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blottingimmunoprecipitated FAP, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.
Western Blot-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -
Western Blot-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti FAP recombinant monoclonal antibody [BLR150J] used at 1:1000. Secondary: HRP conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Immunohistochemistry-Paraffin-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -
Immunohistochemistry-Paraffin-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.
ICC/IF- Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -
ICC/IF- Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.Applications for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 to 1:500
Immunohistochemistry-Paraffin
1:100 to 1:500
Immunoprecipitation
20 µl/1 mg lysate
Multiplex Immunofluorescence
1:100
Western Blot
1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: Fibroblast Activation Protein alpha/FAP
Alternate Names
FAP, Seprase
Gene Symbol
FAP
Additional Fibroblast Activation Protein alpha/FAP Products
- All Products for Fibroblast Activation Protein alpha/FAP
- Fibroblast Activation Protein alpha/FAP cDNA Clones
- Fibroblast Activation Protein alpha/FAP ELISA Kits
- Fibroblast Activation Protein alpha/FAP Lysates
- Fibroblast Activation Protein alpha/FAP Primary Antibodies
- Fibroblast Activation Protein alpha/FAP Proteins and Enzymes
- Fibroblast Activation Protein alpha/FAP Simple Plex
Product Documents for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)
There are currently no reviews for this product. Be the first to review Fibroblast Activation Protein alpha/FAP Antibody (BLR150J) and earn rewards!
Have you used Fibroblast Activation Protein alpha/FAP Antibody (BLR150J)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...