Fibronectin Antibody (DH1)

Novus Biologicals | Catalog # NBP1-51723

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Chicken, Guinea Pig, Rabbit

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DH1
View all Fibronectin Primary Antibodies »

Product Specifications

Immunogen

Purified fibronectin from A8387 fibrosarcoma cells

Marker

Mesenchymal Cells Marker

Specificity

The antibody is specific to extradomain A (EDA) sequence of a cellular fibronectin and recognizes thus only the cellular fibronectin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Fibronectin Antibody (DH1)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, 1% BSA

Preservative

0.1% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Fibronectin

Integrin beta1 also known as integrin VLA-4 beta chain, fibronectin receptor beta chain, and CD29) is a 130 kD integrin beta chain containing VWFA-like domains. Five isoforms have been reported (beta1A-1D, 1C-2). This Type I membrane protein is a subunit of the heterodimeric receptor for laminin, fibronectin, collagen, fibrinogen, VCAM1, cytotactin, osteopontin, epiligrin, thrombospondin, and vitronectin. Signal transduction through the integrins affects embryogenesis, hemostasis, tissue repair, immune response, and metastatic spread of tumor cells. Integrin beta1 can be upregulated by LPS with soluble CD14 and TNF-alpha; downregulation of integrin beta1 by alpha-melanocyte stimulating hormone and progesterone. Integrin beta1 is modified by phosphorylation and glycosylation. Integrin beta1 partners with integrin alpha subunits alphav, and alpha1 through alpha1.1. The Poly6004 antibody recognizes the N-terminal region of human integrin beta1 and has been shown to be useful for Western blotting. Partners with integrin alpha subunits alphav, alpha1 through alpha11.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (DH1)

Certificate of Analysis

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Product Specific Notices for Fibronectin Antibody (DH1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibronectin Antibody (DH1)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fibronectin Antibody (DH1)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 

  • Q: What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

    A: I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NBP1-51723, the dilution range is 1:500-1:1000.

  • Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 

  • Q: What is the dilution factor for IHC for starting? Because the range of dilution factor on the datasheet is so broad, I am confused which dilution factor is suitable for starting.

    A: I can recommend the following dilution to start. Keep in mind that these will all vary greatly by sample type, so these are just suggestions. You may need to use more or less antibody. For catalog number NBP1-51723, the dilution range is 1:500-1:1000.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies