Fibulin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84725

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fibulin 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NTLGSYLCSCSVGFRLSVDGRSCEDINECSSSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGHICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGGFRCLAFECPEN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fibulin 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Analysis in human placenta and skeletal muscle tissues using NBP1-84725 antibody. Corresponding FBLN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human endometrium shows moderate positivity in extracellular matrix.
Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725]

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human skin shows strong positivity in extracellular matrix.
Fibulin 1 Antibody - BSA Free Western Blot: Fibulin 1 Antibody - BSA Free [NBP1-84725]

Western Blot: Fibulin 1 Antibody - BSA Free [NBP1-84725]

Analysis in human cell lines HeLa and SK-MEL-30 using Anti-FBLN1 antibody. Corresponding FBLN1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Applications for Fibulin 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
IHC,HIER pH6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 3 using NBP1-84725 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibulin 1

Fibulin 1 is a matricellular glycoprotein component of the elastic fiber core in connective tissues. It self-aggregates and additionally binds to several extracellular matrix and cellular proteins including Aggrecan, Versican, Fibronectin, ECM1, NOV/CCN3, and pro-HB-EGF. Fibulin 1c enhances ADAMTS-1 mediated cleavage of Aggrecan and Versican and can itself be cleaved by neutrophil elastase or MMP-13. It functions as a bridge between Fibrinogen and Fibrin, enabling Fibrin attachment to the platelet cell surface via Integrin alpha 2 beta 3. Fibulin 1 inhibits cardiomyocyte proliferation and Fibronectin-mediated cell adhesion and can promote bone formation.

Alternate Names

FBLN1, FIBL1

Gene Symbol

FBLN1

Additional Fibulin 1 Products

Product Documents for Fibulin 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fibulin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for Fibulin 1 Antibody - BSA Free

Customer Reviews for Fibulin 1 Antibody - BSA Free (1)

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  • Fibulin 1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human skin
    Species: Human
    Verified Customer | Posted 04/11/2019
    Fibulin 1 Antibody - BSA Free NBP1-84725

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