Filamin B Antibody (GT1282) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13527
![Western Blot: Filamin B Antibody (GT1282) [NBP3-13527]](https://resources.rndsystems.com/images/products/Filamin-B-Antibody-GT1282-Western-Blot-NBP3-13527-img0003.jpg "Western Blot: Filamin B Antibody (GT1282) [NBP3-13527]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (99%), Chicken (97%), Mouse (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT1282
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Filamin B Antibody (GT1282) - Azide and BSA Free
Western Blot: Filamin B Antibody (GT1282) [NBP3-13527]
Western Blot: Filamin B Antibody (GT1282) [NBP3-13527] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT1282] (NBP3-13527) diluted at 1:1000.![Immunohistochemistry-Paraffin: Filamin B Antibody (GT1282) [NBP3-13527]](https://resources.rndsystems.com/images/products/Filamin-B-Antibody-GT1282-Immunohistochemistry-Paraffin-NBP3-13527-img0002.jpg "Immunohistochemistry-Paraffin: Filamin B Antibody (GT1282) [NBP3-13527]")
Immunohistochemistry-Paraffin: Filamin B Antibody (GT1282) [NBP3-13527]
Immunohistochemistry-Paraffin: Filamin B Antibody (GT1282) [NBP3-13527] - Filamin B antibody [GT1282] detects Filamin B protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. Filamin B antibody [GT1282] (NBP3-13527) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Western Blot: Filamin B Antibody (GT1282) [NBP3-13527]](https://resources.rndsystems.com/images/products/Filamin-B-Antibody-GT1282-Western-Blot-NBP3-13527-img0001.jpg "Western Blot: Filamin B Antibody (GT1282) [NBP3-13527]")
Western Blot: Filamin B Antibody (GT1282) [NBP3-13527]
Western Blot: Filamin B Antibody (GT1282) [NBP3-13527] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT1282] (NBP3-13527) diluted at 1:1000.Applications for Filamin B Antibody (GT1282) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Filamin B
Alternate Names
ABP-278filamin B, beta (actin binding protein 278), ABP-280 homolog, actin binding protein 278, Actin-binding-like protein, AOI, beta-filamin, DKFZp686O033, Fh1, filamin B, beta, Filamin homolog 1, Filamin-3, filamin-B, FLN1L, FLN3, FLN-B, Larsen syndrome 1 (autosomal dominant), LRS1, SCT, TABPABP-280, TAPDKFZp686A1668, Thyroid autoantigen, Truncated ABP, Truncated actin-binding protein
Gene Symbol
FLNB
Additional Filamin B Products
Product Documents for Filamin B Antibody (GT1282) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Filamin B Antibody (GT1282) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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