FKBP52/FKBP4 Antibody (Hi52C) - BSA Free

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874]

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) - BSA Free [NB110-96874] -

Melatonin downregulates FKBP4, but is not related to phosphorylation of GR.A–C, F SH-SY5Y cells were treated with melatonin (1 μM) for 30 min and then with cortisol (1 μM) for 24 h. A The expression of p-GR and GR were detected by western blot. Loading control is beta -actin. n = 5. B The expression of GR protein in subcellular fraction samples was detected by western blotting. Lamin A/C and alpha -tubulin were used as a nuclear and cytosolic loading control, respectively. n = 5. C The cells were immunostained with GR (green) and DAPI (blue). Scale bars, 10 μm (magnification, ×1000). n = 5. D, G Mice were injected with melatonin (10 mg/kg) and then with corticosterone (10 mg/kg) for 7 days. D Slide samples for immunohistochemistry were immunostained with GR (green) and DAPI (blue). Scale bars, 140 μm (magnification, ×100). n = 5. E SH-SY5Y cells were treated with melatonin for 30 min and then with cortisol for 12 h. The mRNA expression of regulatory proteins related to cytosolic GR complex, dynein complex, and NPC were analyzed by real time PCR. n = 5. F, G FKBP4 was detected by western blot. Loading control is beta -actin. n = 5. All blots and immunofluorescence images are representative. The representative images were acquired by SRRF imaging system. All data are presented as a mean +/- S.E.M. *p < 0.05 versus control, #p < 0.05 versus cortisol or corticosterone. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36810730), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) - BSA Free [NB110-96874] -

DNMT1-mediated epigenetic downregulation of FKBP4 by melatonin reverses cortisol-induced mitochondrial dysfunction.A, B SH-SY5Y cells were treated with melatonin (1 μM) for 30 min and then with cortisol (1 μM) for 12 h. A Dot blot assay was performed through DNA extraction. The methylation level was confirmed through 5-mc antibody. n = 5. B The methylation status of the CpG region of the FKBP52 was confirmed in extracted gDNA. n = 5. C The cells were transfected with NT siRNA or DNMT1 siRNA for 24 h before melatonin treatment for 12 h. The mRNA expression of FKBP52 were analyzed by quantitative real time PCR. n = 5. D The cells were transfected with NT siRNA or DNMT1 siRNA for 24 h before melatonin treatment for 24 h. FKBP4 levels were detected by western blot. Loading control is beta -actin. n = 5. E The cells were transfected with NT siRNA or DNMT1 siRNA for 24 h before melatonin or cortisol treatment for 24 h. Flow cytometry was used to measure mitochondrial mass by Mitotracker green staining. n = 5. F The cells were transfected with NT siRNA or DNMT1 siRNA for 24 h before melatonin or cortisol treatment for 48 h. Flow cytometry was used to detect mitochondrial membrane potential by TMRE staining. n = 5. G The cells were transfected with NT siRNA or DNMT1 siRNA 24 h before melatonin or cortisol treatment for 72 h. Annexin V/PI staining was performed to detect cell apoptosis. n = 5. All blots and fluorescence images are representative. All data are presented as a mean +/- S.E.M. *p < 0.05 versus control or control + NT siRNA, #p < 0.05 versus melatonin + NT siRNA or cortisol + NT siRNA, and $p < 0.05 versus cortisol and melatonin + NT siRNA. NS means non-staining. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36810730), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FKBP52/FKBP4 Antibody (Hi52C) - BSA Free [NB110-96874] -

Melatonin downregulates FKBP4, but is not related to phosphorylation of GR.A–C, F SH-SY5Y cells were treated with melatonin (1 μM) for 30 min and then with cortisol (1 μM) for 24 h. A The expression of p-GR and GR were detected by western blot. Loading control is beta -actin. n = 5. B The expression of GR protein in subcellular fraction samples was detected by western blotting. Lamin A/C and alpha -tubulin were used as a nuclear and cytosolic loading control, respectively. n = 5. C The cells were immunostained with GR (green) and DAPI (blue). Scale bars, 10 μm (magnification, ×1000). n = 5. D, G Mice were injected with melatonin (10 mg/kg) and then with corticosterone (10 mg/kg) for 7 days. D Slide samples for immunohistochemistry were immunostained with GR (green) and DAPI (blue). Scale bars, 140 μm (magnification, ×100). n = 5. E SH-SY5Y cells were treated with melatonin for 30 min and then with cortisol for 12 h. The mRNA expression of regulatory proteins related to cytosolic GR complex, dynein complex, and NPC were analyzed by real time PCR. n = 5. F, G FKBP4 was detected by western blot. Loading control is beta -actin. n = 5. All blots and immunofluorescence images are representative. The representative images were acquired by SRRF imaging system. All data are presented as a mean +/- S.E.M. *p < 0.05 versus control, #p < 0.05 versus cortisol or corticosterone. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36810730), licensed under a CC-BY license. Not internally tested by Novus Biologicals.