FKBP52/FKBP4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33702

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FKBP52 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FKBP52/FKBP4. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FKBP52/FKBP4 Antibody - BSA Free

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FKBP4 antibody.
Immunocytochemistry/ Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-33702]

Immunocytochemistry/ Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-33702]

Immunocytochemistry/Immunofluorescence: FKBP52/FKBP4 Antibody [NBP1-33702] - Paraformaldehyde-fixed A431, using (NBP1-33702) antibody at 1:200 dilution.
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug GL261 whole cell lysate/extract, B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702]

Western Blot: FKBP52/FKBP4 Antibody [NBP1-33702] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.

Applications for FKBP52/FKBP4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FKBP52

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin Abinding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share approx. 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90- binding characteristics (3, 5). Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen and glucocorticoid receptors (5).

Long Name

52 kDa FK506 Binding Protein

Alternate Names

FKBP4, FKBP59, HBI, Hsp56

Gene Symbol

FKBP4

Additional FKBP52 Products

Product Documents for FKBP52/FKBP4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FKBP52/FKBP4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Phosphatases and Regulators

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Protocols

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