Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FLIP Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP. Amino Acid Squence: SAEVIHQVEEALDTDEK

Specificity

FLIP recognizes all FLIP splice variants including FLIPa, b, and y.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FLIP Antibody - BSA Free

Western Blot: FLIP AntibodyBSA Free [NBP1-77016]

Western Blot: FLIP AntibodyBSA Free [NBP1-77016]

Western Blot: FLIP Antibody [NBP1-77016] - Analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 ug/ml.
Western Blot: FLIP AntibodyBSA Free [NBP1-77016]

Western Blot: FLIP AntibodyBSA Free [NBP1-77016]

Western Blot: FLIP Antibody [NBP1-77016] - K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.
FLIP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77016] -

Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77016] - Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 u/mL.
FLIP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77016] -

Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77016] - Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 ug/mL.
FLIP Antibody - BSA Free

Immunohistochemistry: FLIP Antibody - BSA Free [NBP1-77016] -

Immunohistochemistry: FLIP Antibody - BSA Free [NBP1-77016] - Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 u/mL.
FLIP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77016] -

Immunocytochemistry/ Immunofluorescence: FLIP Antibody - BSA Free [NBP1-77016] - Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 ug/ml.

Red: FLIP Antibody
Blue: DAPI staining

Applications for FLIP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FLIP

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.

Long Name

FLICE Inhibitory Proteins

Alternate Names

CASPER, CFLAR, FLAME-1, I-FLICE

Gene Symbol

CFLAR

UniProt

AAC51622 (Human)

Additional FLIP Products

Product Documents for FLIP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FLIP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FLIP Antibody - BSA Free

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Protocols

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FAQs

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