FMO5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86093

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FMO5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLGAIMPISELQGRWATQVFKGLKTLPSQSEMMAEISKAQEEIDKRYVESQRHTIQGDYIDTMEELADLVGVRPNLLSLAFTDPKLALHLLLGPCTPIHYRVQGPGKWDGARKAILTTDD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FMO5 Antibody - BSA Free

Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093]

Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093]

Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093] - Staining in human liver and cerebral cortex tissues using anti-FMO5 antibody. Corresponding FMO5 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: FMO5 Antibody [NBP1-86093]

Immunocytochemistry/ Immunofluorescence: FMO5 Antibody [NBP1-86093]

Immunocytochemistry/Immunofluorescence: FMO5 Antibody [NBP1-86093] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093]

Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093]

Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093]

Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093]

Immunohistochemistry-Paraffin: FMO5 Antibody [NBP1-86093] - Staining of human cerebral cortex shows low expression as expected.

Applications for FMO5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FMO5

FMO5, also known as Dimethylaniline monooxygenase [N-oxide-forming] 5, has a 533 amino acid long isoform that is 60 kDa and a short 285 amino acid isoform that is 32 kDa; is most often expressed in adult and fetal liver, and in contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme. This protein has been studied for its involvement in trimethylaminuria, hearing loss, hepatitis, and atrioventricular septal defect. FMO5 interacts with CYP3A5, CYP3A43, CYP2D6, CYP3A7, and CYP3A4 and it has been linked to Busulfan Q06828 (pharmacodynamics) and drug metabolism - cytochrome P450 pathways.

Alternate Names

dimethylaniline monooxygenase [N-oxide-forming] 5, Dimethylaniline oxidase 5, EC 1.14.13.8, flavin containing monooxygenase 5, FMO 5, Hepatic flavin-containing monooxygenase 5

Gene Symbol

FMO5

Additional FMO5 Products

Product Documents for FMO5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FMO5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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