FOXI1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16518

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human FOXI1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: ZebraFish (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FOXI1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody [NBP2-16518]

Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody [NBP2-16518]

Immunocytochemistry/Immunofluorescence: FOXI1 Antibody [NBP2-16518] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: FOXI1 Antibody [NBP2-16518]

Western Blot: FOXI1 Antibody [NBP2-16518]

Western Blot: FOXI1 Antibody [NBP2-16518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FOXI1 Antibody [NBP2-16518]

Western Blot: FOXI1 Antibody [NBP2-16518]

Western Blot: FOXI1 Antibody [NBP2-16518] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: FOXI1 Antibody [NBP2-16518]

Immunohistochemistry-Paraffin: FOXI1 Antibody [NBP2-16518]

Immunohistochemistry-Paraffin: FOXI1 Antibody [NBP2-16518] - Huh7 xenograft, using FOXI1 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FOXI1 Antibody - BSA Free

Western Blot: FOXI1 Antibody [NBP2-16518] -

Western Blot: FOXI1 Antibody [NBP2-16518] -Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
FOXI1 Antibody - BSA Free

Immunohistochemistry: FOXI1 Antibody [NBP2-16518] -

FOXI1 antibody [N2C3] detects FOXI1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
FOXI1 antibody [N2C3] (NBP2-16518) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FOXI1 Antibody - BSA Free

Western Blot: FOXI1 Antibody [NBP2-16518] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] (NBP2-16518) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for FOXI1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FOXI1

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

FKHL10HFH3, forkhead box I1, forkhead box protein I1, forkhead-like 10, forkhead-related activator 6, Forkhead-related protein FKHL10, Forkhead-related transcription factor 6, FREAC-6, FREAC6FKH10, Hepatocyte nuclear factor 3 forkhead homolog 3, HFH-3, HNF-3/fork-head homolog 3, HNF-3/fork-head homolog-3, MGC34197

Gene Symbol

FOXI1

UniProt

Additional FOXI1 Products

Product Documents for FOXI1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FOXI1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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