FoxJ1/HFH4 Antibody (CL3989) - BSA Free

Novus Biologicals | Catalog # NBP2-59032

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3989

Format

BSA Free
View all FoxJ1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q92949, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FoxJ1/HFH4 Antibody (CL3989) - BSA Free

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Analysis in human fallopian tube and skeletal muscle tissues using NBP2-59032 antibody. Corresponding FOXJ1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunocytochemistry/ Immunofluorescence: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunocytochemistry/Immunofluorescence: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Staining of BEWO cells using the Anti-FOXJ1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Staining of human tonsil shows no positivity in germinal center cells as expected.
Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Staining of human endometrium shows strong nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for FoxJ1/HFH4 Antibody (CL3989) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2000

Immunohistochemistry-Paraffin

1:1000 - 1:2000
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxJ1

FOXJ1 is a member of the forkhead gene family, which was originally identified in Drosophila. The forkhead family is composed of transcription factors with a conserved 100-amino acid DNA-binding motif.[supplied by OMIM]

Long Name

Forkhead Box J1

Alternate Names

FKHL13, HFH-4

Gene Symbol

FOXJ1

Additional FoxJ1 Products

Product Documents for FoxJ1/HFH4 Antibody (CL3989) - BSA Free

Certificate of Analysis

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Product Specific Notices for FoxJ1/HFH4 Antibody (CL3989) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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