FoxK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83109

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FoxK1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GVPGHTVTILQPATPVTLGQHHLPVRAVTQNGKHAVPTNSLAGNAYALTSPLQLLATQASSSAPVVVTRVCEVGPKEPAAAVAATATTTPATATTASASASSTGEPEVKRSRVEEPSGAVTTPAG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FoxK1 Antibody - BSA Free

Western Blot: FoxK1 Antibody [NBP1-83109]

Western Blot: FoxK1 Antibody [NBP1-83109]

Western Blot: FoxK1 Antibody [NBP1-83109] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: FoxK1 Antibody [NBP1-83109]

Immunocytochemistry/ Immunofluorescence: FoxK1 Antibody [NBP1-83109]

Immunocytochemistry/Immunofluorescence: FoxK1 Antibody [NBP1-83109] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109] - Analysis in human cerebral cortex and liver tissues using NBP1-83109 antibody. Corresponding FOXK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109] - Staining of human cerebral cortex, liver, lymphoid tissues and testis using Anti-FOXK1 antibody NBP1-83109 (A) shows similar protein distribution across tissues to independent antibody NBP1-83110 (B).
Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109] - Staining of human lymph node shows moderate to strong nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109]

Immunohistochemistry-Paraffin: FoxK1 Antibody [NBP1-83109] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
FoxK1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: FoxK1 Antibody - BSA Free [NBP1-83109]

Chromatin Immunoprecipitation-exo-Seq: FoxK1 Antibody - BSA Free [NBP1-83109]

ChIP-Exo-Seq composite graph for Anti-FOXK1 (NBP1-83109) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for FoxK1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxK1

Forkhead box protein K1 (FoxK1) is a member of the human Forkhead-box (FOX) family of proteins. The Forkhead-box domain is a highly conserved DNA binding domain of about 100 amino acids found in proteins that function as transcriptional regulators. FoxK1 also bears an FHA (forkhead-associated) domain, a putative nuclear signaling domain that binds phospho-peptides. FoxK1 has been shown to play a role in the regulation of myogeneic progenitor cells and skeletal muscle regeneration.

Long Name

Forkhead Box K1

Alternate Names

MNF

Gene Symbol

FOXK1

Additional FoxK1 Products

Product Documents for FoxK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FoxK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Muscle Stem Cell Markers

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Protocols

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FAQs

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