FOXL2 Antibody

Novus Biologicals | Catalog # NB100-1277

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Ferret

Cited:

Human, Mouse, Avian - Chicken, Bovine, Ferret

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FOXL2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DSKTGALHSRLDL corresponding to C-Terminus according to NP_075555.1.

Reactivity Notes

Use in Bovine, Human, Mouse reported in scientific literature (PMID: 33956944). Ferret reactivity reported in scientific literature (PMID: 25289806).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FOXL2 Antibody

FOXL2 Antibody

Western Blot: FOXL2 Antibody [NB100-1277] -

Western Blot: FOXL2 Antibody [NB100-1277] - (1ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody [NB100-1277]

Immunocytochemistry/ Immunofluorescence: FOXL2 Antibody [NB100-1277]

Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF image submitted by a verified customer review.
FOXL2 Antibody

Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] -

Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - (8ug/ml) staining of paraffin embedded Human Ovary. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
FOXL2 Antibody

Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] -

Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.

Applications for FOXL2 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

8 ug/mL

Immunohistochemistry Whole-Mount

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx. 50kDa band observed in Mouse Ovary lysates and in preliminary testing of Human Uterus and Rat Ovary lysate (calculated MW of 38.9kDa according to Mouse NP_036150.1). This molecular weight is routinely observed by other sources. Primary incubation 1 hour at room temperature.

IHC-WhMt usage reported in scientific literature (PMID: 24753105). ICC/IF reported in scientific literature (PMID: 27698028). IHC-Fr reported in scientific literature (PMID: 23091613). ChIP reported in scientific literature (PMID: 30212841). IHC-P reported in scientific literature (PMID: 30304016).

Reviewed Applications

Read 1 review rated 5 using NB100-1277 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FOXL2

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

Long Name

Forkhead Box L2

Alternate Names

BPES, BPES1, PFRK, PINTO, POF3

Entrez Gene IDs

668 (Human); 26927 (Mouse)

Gene Symbol

FOXL2

UniProt

P58012

Additional FOXL2 Products

Product Documents for FOXL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FOXL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FOXL2 Antibody (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • Name: Ripla Arora
    Application: Immunofluorescence
    Sample Tested: Mouse Ovary
    Species: Mouse
    Verified Customer | Posted 07/17/2015
    E14.5 mouse ovary section stained with Foxl2 (magenta), germ cell marker (green) and hoechst (grey)
    FOXL2 Antibody NB100-1277

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Protocols

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